EMAGE mouse gene expression spatial database

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EMAGE:32094

Slc39a4 solute carrier family 39 (zinc transporter), member 4 ( MGI:1919277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003030_01	euxassay_003030_02	euxassay_003030_03	euxassay_003030_04	euxassay_003030_05

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euxassay_003030_11	euxassay_003030_12	euxassay_003030_13	euxassay_003030_14	euxassay_003030_15

euxassay_003030_16	euxassay_003030_17	euxassay_003030_18	euxassay_003030_19	euxassay_003030_20

euxassay_003030_21	euxassay_003030_22	euxassay_003030_23	euxassay_003030_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

moderate

regional

moderate expression: see section 08 09 20 21

upper jaw molar

moderate

regional

moderate expression: see section 08 20 21

liver lobe

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 13 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 13 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4656
Entity Detected:	Slc39a4, solute carrier family 39 (zinc transporter), member 4 ( MGI:1919277)
Sequence:	sense strand is shown >T4656 CTCCAGTCTGGCCCCGGACAGTCCCAGCTGTCTCCAGCTAGCCCAGAAGTCAGCACCTCTACAAGGAACG CTTTTGGGGGCCTGAGGCGTGATGCTCCCAAAGTCGGTCACACAGGGACTTGTGTTGGCTCTGCTGGTGG GCACAGTGGCAGTGGCCCGGCCCAGGAACCTGCTCAGCCTGCTCGCCTTGGGCCAGGGTGCTCTGGATCG CCTGGAACTGGACGGCCTGTTAAATACGCTGGTGGCCCGTGTGCACTGCACCGACGGGCCGTGTGAAAAG TGTCTGTCTGTGGAGAATGTCTTGGCTCTAGGCAAACCTGACAAGCCACAGCCTGCCCCAGAATCAGTCC TGGAGTCCAGACACATTATTTACCTTAGTGCTGCTGCTGCCCTCTACCTTAACAACCCAGAGAAAACATG CAAGGACATCCAAGCTGGCCTCTTGGCCTCCCATGTGGACGATTACCTGGCCACACTGGAGAGTCCAGAG GCCATGACCCTGGGTCTGAGCCAGCTACTGCAGAAGATTGAGGCCCATGCTGCCAGCCAACCCACCGGGG AGAAGACCTGTGTAGATCTTCCCCAACTGCTGGAGGAGGCTGAGGCAGCAGGGGTTTCCAAAAGCGCCGG CCTGGTCTTGACTGCCTTGCTGGATCATGTCATTAATGGGTCCTGCTTCCAAGGCCTGCCTAGCCCTCAG TACTTTGTGGACTTTGTGTTCAGGCTACACAGTAGTGACCCTCCCAATATCACGCTGCATGAACTGGAGA ATTTGATGCATCACCTTGGGGTGGGTGGAGAGGACCACAGTGACCATGATGACCACGGTGATCATGCTGA CCACAGTCATCCGGACAGGAAAGCCAGCCACCAAGACTCTGAGCTCCATACTCCCCACAACAGCAACTCT AGTGTATGGGACACGCTGTGCCTGAGTGCCAAAGATATAATGGCTGTGTATGGGCTATCTGAAGAGGCTG GGGTGAGCCCTCAGGCCTGGGCCCAACTGACCCCTGCCTTGGTCCAGCAGCAGCTAAGTGGAGCCTGCAG CCCCTACCCCACTATCCGTATTCAGGACCAGCTCAGTCAAACAGAGAGGTATCTCTATGGCTCGCTGGCC ACCCTGCTCATCTGCCTCTGTGCTGTGTTCGGTCTTCTGCTGCTGACCTGTGCCAAATGCAGCACAGCCA CCCACTACATCATGCAGACCTTCCTAAGCTTGGCTGTGGGCGCACTTACCGGCGATGCTCTTCTGCACCT GATACCCAAGGTGCTGGGACTGCACACACATGGTGGAGAGGGTCACACCCATGAGGAGGAGGTGGGCGTT GGTGGGCAGGCCACCTGGCGCCTGCTGGCTGTACTTGGAGGCTTCTACATCTTCTTCCTGTTTGAGAGCT TCTTCAACCTCTTGTTGCCCAGGGACCAGGATTCTGAGAAAGATGGGCCTTGTAGCCATGGTGGGCACAG CCATGGAATATCTCTGCAGCTGGCACCAAGCAATCTCCGACAGTCCAAACAGACCCATGAAAGCTCTCGT TCAGACTTGGTGGCAGAGGAGACCCCGGAACTACTGAACCCAGAGACCCGGCGACTGAGAGCAGAGCTGA GACTGTTGCCCTATCTGATCACACTGGGCGACGCGGTACACAACTTCGCTGACGGGCTCGCTGTGGGCGC CGCCTTCTCATCCTCGTGGAAGACTGGGCTGGCCACTTCATTGGCGGTGTTCTGTCATGAGCTGCCCCAT GAACTCGGGGACTTCGCTGCTCTGCTGCATGCCGGGCTGAGTGTGAAGCGTGCGCTTTTGCTGAATCTGG CCTCAGCGCTCACAGCATTCGCAGGCCTCTACGTGGCTCTAGCAGTCGGAGTAGGCGAGGAGGGCGAGGC TTGGATTCTGGCGGTAGCAACCGGCCTCTTCCTTTACGTGGCGCTTTGTGACATGCTCCCAGCCATGATG AATGTGCGCGACCAGCGGCCCTGGCTTCTTTTCCTGCTCCACAACGTGGGTCTGCTGGGCGGCTGGACCG TCCTGCTGCTGCTGTCATTGTACGAAGACAACATCACCTTCTGACAGCTCTATCCCATCCCAGTCCTTGT CCCTGTCTGTTGACTCTGCTTTACCTTCTTAAGCCACCTAATTGTTGGCCCCACTACGGGTAGCCAGAGG CTTTGAGCCTCATTTCCTTGCTCTGACTTCAATAAAGACTTTTCAATCAAACCCAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5011088 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5011088 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003030 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source