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EMAGE:32097

Thbd thrombomodulin ( MGI:98736)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097
euxassay_003032_01 euxassay_003032_02 euxassay_003032_03 euxassay_003032_04 euxassay_003032_05
EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097
euxassay_003032_06 euxassay_003032_07 euxassay_003032_08 euxassay_003032_09 euxassay_003032_10
EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097
euxassay_003032_11 euxassay_003032_12 euxassay_003032_13 euxassay_003032_14 euxassay_003032_15
EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097 EMAGE:32097
euxassay_003032_16 euxassay_003032_17 euxassay_003032_18 euxassay_003032_19 euxassay_003032_20
EMAGE:32097 EMAGE:32097 EMAGE:32097
euxassay_003032_21 euxassay_003032_22 euxassay_003032_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindlimb digit 5 mesenchyme
moderate moderate
regionalmoderate expression: see section 05 06 23
knee mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
midbrain meninges
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
hindlimb digit 4 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05 06 23
rectum
moderate moderate
regionalmoderate expression: see section 15 16
axial skeleton sacral region
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19
axial skeleton tail region
strong strong
regionalstrong expression: see section 13 14 15 16 17
telencephalon meninges
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
nose
strong strong
regionalstrong expression: see section 10 11 16 17 18
left lung
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15
lower leg mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03
midgut loop
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 08
diaphragm
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 17 18 19 weak expression: see section 20
trachea
strong strong
regionalstrong expression: see section 14 15
hindlimb digit 3 mesenchyme
moderate moderate
regionalmoderate expression: see section 03 04 05 23
upper leg mesenchyme
moderate moderate
regionalmoderate expression: see section 03
femur
strong strong
regionalstrong expression: see section 08 09 21 22 23
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 08 21 22
spinal cord meninges
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20
hindgut
moderate moderate
regionalmoderate expression: see section 14 15 16
esophagus epithelium
strong strong
regionalstrong expression: see section 14 15 16
mandible
strong strong
regionalstrong expression: see section 04 05 06 07 08 19 20 21 22 23 moderate expression: see section 09 10 18
tongue epithelium
strong strong
regionalstrong expression: see section 11 12 14 15 16 moderate expression: see section 13
right lung
strong strong
regionalstrong expression: see section 16 17 18 19 20 21 22 23
thoracic intervertebral disc
strong strong
regionalstrong expression: see section 18
hindlimb digit 1 mesenchyme
moderate moderate
regionalmoderate expression: see section 03 04 23
lumbar intervertebral disc
strong strong
regionalstrong expression: see section 18
stomach
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 03 04 05 06 07 08
shoulder mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
diencephalon meninges
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21
urethra of male
strong strong
regionalstrong expression: see section 13 14 15 16
hindlimb digit 2 mesenchyme
moderate moderate
regionalmoderate expression: see section 03 04 23
tongue muscle
strong strong
regionalstrong expression: see section 12 15 16 moderate expression: see section 13
midgut
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 19 20 21
hindbrain meninges
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
hand mesenchyme
moderate moderate
regionalmoderate expression: see section 03
cervical intervertebral disc
strong strong
regionalstrong expression: see section 18
forearm mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
maxilla
strong strong
regionalstrong expression: see section 04 05 06 07 08 19 20 21 22 moderate expression: see section 09 10 18
nucleus pulposus
strong strong
regionalstrong expression: see section 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4837
Entity Detected:Thbd, thrombomodulin ( MGI:98736)
Sequence:sense strand is shown

>T4837
CCACGCGTCCGGCAGGGACTAGGAGCCTGGGCTAGAAACGTCTAGGTTGTGATAGAGGCTAGCTGCTGTC
GCCGCAGGCATACGGAAACCCCTCTGCCTGGACTGGGTGTCTGCACCAGCGCAGGGCTAGCTTTTCTCGG
AACTTGCACGGTGCACCCCTGGACAGCATGCTTGGGATTTTCTTTCTGGGTGTGCTGGCTCCAGCTAGCC
TGGGGCTCTCCGCACTAGCCAAGCTGCAGCCCACAGGCAGTCAATGCGTGGAGCATGAGTGCTTCGCGCT
TTTCCAGGGCCCCGCGACCTTCCTCGATGCCAGCCAGGCCTGCCAGCGCCTGCAAGGACATTTGATGACA
GTGCGCTCCTCAGTGGCTGCCGATGTCATCTCCCTTCTGCTGAGCCAGAGCAGTATGGATTTAGGGCCCT
GGATCGGTTTACAGCTCCCGCAGGGCTGTGACGACCCGGTGCATCTCGGGCCCCTGCGCGGCTTCCAGTG
GGTTACTGGCGATAACCACACCAGTTACAGCAGGTGGGCGCGGCCCAACGACCAGACGGCTCCACTCTGC
GGCCCTCTGTGCGTCACGGTCTCGACAGCAACTGAAGCTGCACCCGGCGAGCCGGCCTGGGAAGAGAAGC
CATGCGAGACTGAGACCCAGGGTTTCCTCTGTGAGTTTTACTTCACAGCTTCCTGCAGGCCTCTGACGGT
GAATACTCGCGATCCTGAGGCTGCCCACATCTCTAGTACCTACAACACCCCGTTCGGGGTCAGTGGTGCG
GACTTTCAAACGCTGCCGGTAGGCAGTTCCGCCGCGGTGGAGCCCCTTGGCTTGGAGCTGGTGTGCAGGG
CCCCGCCCGGAACTTCAGAGGGACACTGGGCTTGGGAAGCGACAGGAGCCTGGAATTGCAGCGTGGAGAA
TGGTGGCTGTGAGTACTTGTGCAATAGGAGCACGAATGAACCCAGATGCCTCTGCCCCAGAGACATGGAC
CTGCAGGCCGATGGACGTTCGTGTGCAAGACCTGTGGTTCAATCGTGCAACGAACTCTGCGAGCATTTTT
GTGTCAGCAACGCTGAAGTGCCAGGCTCTTACTCCTGTATGTGTGAGACAGGCTACCAGTTGGCTGCAGA
CGGACACCGGTGTGAGGACGTGGATGACTGTAAGCAGGGGCCCAATCCATGTCCCCAGCTCTGTGTTAAC
ACCAAGGGCGGCTTCGAATGCTTCTGCTATGATGGCTATGAGTTGGTGGATGGAGAGTGCGTGGAGCTTC
TGGATCCGTGTTTCGGATCTAACTGCGAGTTTCAGTGCCAGCCAGTGAGCCCCACCGACTACCGATGCAT
CTGCGCTCCGGGCTTCGCACCCAAGCCGGATGAACCGCACAAGTGCGAAATGTTCTGCAATGAAACTTCG
TGCCCAGCAGACTGTGACCCTAACTCTCCTACTGTTTGTGAATGCCCTGAAGGCTTCATCCTGGACGAGG
GTTCCGTATGCACGGACATTGATGAGTGCAGTCAAGGTGAATGCTTCACCAGTGAATGTCGAAACTTCCC
TGGCTCCTATGAGTGTATCTGCGGGCCTGACACAGCCCTTGCTGGTCAGATTAGCAAAGACTGTGACCCC
ATCCCTGTTAGGGAAGACACCAAGGAAGAGGAGGGCTCTGGGGAGCCTCCAGTCAGCCCTACGCCAGGCT
CTCCGACAGGTCCCCCTTCTGCAAGGCCAGTGCACTCTGGCGTGCTCATTGGCATCTCCATTGCCAGCCT
GTCCCTGGTGGTGGCGCTTTTGGCGCTTCTCTGTCACCTGCGCAAGAAGCAGGGCGCTGCTCGCGCAGAG
CTGGAGTACAAGTGCGCATCTTCCGCCAAGGAGGTAGTGCTGCAGCACGTCAGGACTGATCGGACGCTGC
AGAAGTTCTGAGGGATTTGCTCCAGAGACCCAGGTGGCCTTTGTCTTTCCGGGCTCTGTACCTCTCCTCT
CCTCTCTCTCTCTTCAGCCTCCCAGCTGTGTTCTCTGGCAACTTAAAAGCATCCTGGCTGGTATAATAAC
CAGAGAAGAGCCCATCCTCTCAGGACAAGCGAGGGTAGGGAGGACTGGAAGCAGGACAGCCCAGTTTCTT
CCAAGTAGATACTGGACAACTGGGCGGAGGTGGCAAATACAGCGGAGATCCCAGAGTACCCCAGTCCCTC
ACCTCACCTCCTAGTGCTGCTGATCTGTAGGCTTGAAGGCAAACCTTGACCCCATGGGCTGGAGATGACC
CAGATATTTATTTTTTTTAAAAGTATTTAGTATTTTCTTCCCTCCAGTTTTCTTCTGCTTGTAAGTCTCC
AGCCCCCCACCGCTTTCTCAGTCCCTCCATTCCCCCCTCCTGTCATTCTCCTCCCCAAACCTGATCATAA
CTTTGCCCTTACCGTTGTTTCCAAACTCTTATGTGAAACAGAAAATAACACTAAAAGCAGAAAACGTTCT
TTTTCACTGGCTTTGGGTATTTAGTCAGAAATTTCAGGTAACCAAAGCAAAAGAATTTTAACAAAAGCTA
AAATATTTCAGCTGAACACTAACTAGTCAATAGTGCTGGAATGTCACAGAAAATAAACTTAAGGAAGTAG
GGTTTTTTTTTTGAAATCTTTGTTTTTGAAAAGGGTGAGCCTGGGTTTTATGATTGTTGCTGTTGTTTTG
AATGGGAATGACAAAAGAGGTCATTATTGTTAAGATTTTTATGCAGGCTCTACAGTGTTATTAATTTTTG
ACAGTGTTCAAAATGTGCAGAGGATCCTTTGTCCAACCCTTTGACATGACAATAGGACATTGCTATCTTC
AGACATACTGGGCCACATTCATAGCTTTCCAAGGATGTATGTGGTCCTGCCTCAACATATCAGAGACTGA
CAGATGGAAGCATCTTCCAGTAAAGCATGAGTTGTGTGCTTCGTGCCGAGCTGACTCTCAACTGTGCTTG
TCCCTTGTAGTCCCGAAATACAAGCAATGTGCTGCTTAGGGAAACATGGAAACTTGGGAATGGAGTCTGG
GGGTGCCTAGATGGGGCTTTCTTTTAATGAGACTCTTGAACAATATCTCGTAATTCAGAGGGATCTTCTA
GCCCTGGCCACTGGCCTGTACACAAGAATTGGGACCTCGCTTGGGATCTGGCTAGAATTGCAAAATCCTA
GCCCCCACCCCTGCCCCACCCCAGTGTGCCAGTTCATAAGAATCTGCATTTTGACAACATCCACAGGGAC
ATTGTCCAGTCATTTCAGGACAACTGGTCTTAAGAGTTTCCAACCTTTGTAGAACATTTAAATGTCGGTT
AATAATAAGTAGCAGGCCATGTTAAGGCCATTTATTATCAAGAAACTGAGGAATTTTCTCTGCATAGCTT
TGCTTTCTGGATACAATAAAATGAGAAGGTACACACCTCTAGATAGTGCCACACAGAGTCCAGAAGGGTT
TTGTTTTAAGTAAGCTAGGAATGAGTTCATATGTTAGTGTAAGGAACAAATGTATTATATGTGTATCTTT
TGTAAAGAAAGGTTTTTCTTTACGGTTTTGTAAGCTCAGCATATTTGTACATATTTATTTATTGGAGTTT
CGCTAGAACACAAGCAAAGCCTTTGCTTATGACGTCACATGTACAAAATAAATAGATGACAGTGTAAAAA
AAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5065481 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065481 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003032 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS