EMAGE mouse gene expression spatial database

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EMAGE:32100

Itpr1 ( MGI:96623)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006317_01	euxassay_006317_02	euxassay_006317_03	euxassay_006317_04	euxassay_006317_05

euxassay_006317_06	euxassay_006317_07	euxassay_006317_08	euxassay_006317_09	euxassay_006317_10

euxassay_006317_11	euxassay_006317_12	euxassay_006317_13	euxassay_006317_14	euxassay_006317_15

euxassay_006317_16	euxassay_006317_17	euxassay_006317_18	euxassay_006317_19	euxassay_006317_20

euxassay_006317_21	euxassay_006317_22	euxassay_006317_23	euxassay_006317_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

moderate

regional

moderate expression: see section 09 10 11 17 18 19 weak expression: see section 07 08 12 15 16

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 02 03 04 09 10 11 12 17 18 19 weak expression: see section 05 06 07 08 13 14 15 16

diencephalon roof plate

weak

regional

weak expression: see section 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35010
Entity Detected:	Itpr1, ( MGI:96623)
Sequence:	sense strand is shown >T35010 ATATTACACCACAGAGCGGGATGAGCAAGGTAGCAAGATCAATGACTTCTTCCTGCGCTCCGAGGACCTC TTTAACGAGATGAACTGGCAGAAGAAACTTCGAGCCCAGCCTGTCTTGTACTGGTGTGCCCGAAACATGT CTTTCTGGAGCAGCATCTCCTTCAACCTGGCCGTCCTGATGAACCTGCTGGTGGCGTTTTTCTATCCATT TAAAGGAGTGAGGGGAGGAACACTAGAGCCACACTGGTCAGGCCTCCTGTGGACAGCCATGCTCATCTCT CTGGCCATTGTCATTGCTCTGCCCAAGCCCCACGGCATCCGGGCCTTAATTGCTTCTACAATCCTACGAC TGATATTTTCAGTTGGGTTGCAGCCCACACTGTTTCTGCTGGGAGCTTTCAATGTCTGCAATAAAATCAT CTTCCTGATGAGCTTTGTGGGCAACTGTGGGACCTTCACCAGAGGCTACCGGGCCATGGTTCTGGATGTG GAGTTCCTCTATCATTTGCTGTATCTACTCATCTGTGCCATGGGCCTCTTCGTACATGAGTTCTTCTATA GCTTGCTGCTTTTTGATTTAGTGTACAGAGAGGAGACTTTGCTTAATGTCATTAAAAGTGTCACCCGCAA TGGACGGTCCATCATCTTGACAGCGGTCCTGGCTCTGATCCTGGTTTACCTGTTCTCAATTGTGGGCTAT CTGTTCTTCAAGGATGACTTTATCTTGGAAGTAGATAGGTTGCCCAATGAAACAGCTGTTCCAGAAACTG GCGAGAGTTTGGCCAACGATTTCCTGTACTCTGATGTGTGCAGGGTAGAGACGGGGGAGAACTGCACCTC TCCTGCACCCAAAGAAGAGCTGCTCCCTGCCGAAGAAACGGAACAGGATAAGGAACACACGTGTGAGACC CTGCTCATGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50105. Forward Primer - name:050105_F_cDNA_Itpr1, sequence:ATATTACACCACAGAGCGGGAT; Reverse Primer - name:050105_N_SP6_cDNA_Itpr1, sequence:CACATGAGCAGGGTCTCACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006317 same experiment
	EMAGE:31639 same embryo
	EMAGE:31616 same embryo
	EMAGE:31572 same embryo
	EMAGE:31573 same embryo
	EMAGE:30469 same embryo
	EMAGE:30898 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source