EMAGE mouse gene expression spatial database

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EMAGE:32113

C1s complement component 1, s subcomponent ( MGI:1355312)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003047_01	euxassay_003047_02	euxassay_003047_03	euxassay_003047_04	euxassay_003047_05

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euxassay_003047_11	euxassay_003047_12	euxassay_003047_13	euxassay_003047_14	euxassay_003047_15

euxassay_003047_16	euxassay_003047_17	euxassay_003047_18	euxassay_003047_19	euxassay_003047_20

euxassay_003047_21	euxassay_003047_22	euxassay_003047_23	euxassay_003047_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 21 22 23 24

kidney calyx

moderate

regional

moderate expression: see section 09 10 11 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4869
Entity Detected:	C1s, complement component 1, s subcomponent ( MGI:1355312)
Sequence:	sense strand is shown >T4869 ATCAGCTCCTGAAGGTAACAACTTTAAAGAGCAAGCCTTAAAGAGAGAAGCTTTGATGAAGGAAGAGGGA AAGACAAGGAACCATGTACAGTGACACTGAGCCCTGGGTGGTCCCTCACTACCAAAGCAGGCACCTGTAC TTGTCAGATAAAGACAGCCAGGACTGCTGACTGCCCTTTACCTCCAAAATCAGAGAGCCAGAGAGCCAGA GAGCCATGGGCAAATCGCCAGGGATGTGGTGTTTGGTCCTGTTTTCTCTTTTGGCATCATTTTCTGCTGA GCCCACCATGCATGGGGAGATCCTGTCCCCTAACTATCCCCAGGCGTACCCCAATGACGTCGTGAAATCT TGGGACATTGAAGTCCCAGAGGGGTTTGGGATTCACCTCTACTTTACCCATGTGGACATAGAGCCGTCAG AGAGCTGTGCATACGACTCAGTGCAGATAATCTCAGGAGGCATCGAGGAAGGGAGACTCTGTGGCCAGAA GACCAGCAAGAGCCCCAACTCCCCCATTATAGAAGAGTTTCAGTTCCCATACAATAAACTTCAGGTGGTC TTTACCTCAGACTTCTCCAACGAAGAACGGTTTACGGGCTTTGCAGCATACTACACTGCCATAGACATAA ATGAATGCACAGATTTTACAGATGTCCCCTGTAGCCACTTCTGCAATAACTTCATTGGTGGATACTTCTG CTCCTGTCCCCCAGAATACTTCCTCCACGATGACATGAGGAATTGTGGAGTCAACTGTAGTGGAGATGTG TTCACTGCCCTGATTGGGGAGATCTCAAGTCCCAATTATCCCAATCCATACCCGGAGAACTCAAGGTGTG AATACCAGATTCAGCTGCAGGAGGGCTTCCAAGTGGTGGTGACGATGCAAAGAGAAGATTTTGATGTGGA GCCTGCTGACTCAGAGGGGAACTGCCCTGACAGTTTAACTTTTGCTTCAAAAAACCAACAGTTTGGTCCT TACTGTGGTAATGGGTTCCCTGGACCGCTAACTATTAGAACCCAGAGCAATACTCTCGGCATTGTCTTTC AAACTGACCTAATGGGGCAAAAGAAGGGTTGGAAGCTTCGTTACCATGGAGATCCCATCTCCTGTGCCAA AAAAATCACTGCCAATTCTACTTGGGAGCCCGATAAGGCAAAATACGTGTTTAAAGATGTGGTGAAGATA ACCTGTGTGGACGGATTTGAAGTTGTGGAGGGACATGTTAGCTCTACTTCATACTATTCCACTTGTCAAA GCGATGGACAGTGGAGCAACTCCGGTCTAAAATGTCAACCTGTGTATTGTGGAATTCCAGATCCCATTGC GAATGGTAAAGTTGAAGAGCCAGAGAATAGTGTGTTTGGTACTGTTGTTCACTACACGTGTGAAGAGCCA TATTACTACATGGAACATGAAGAAGGTGGGGAGTATCGATGTGCTGCAAATGGGAGATGGGTAAATGACC AGCTGGGTATTGAGCTTCCAAGATGTATTCCAGCCTGTGGAGTACCCACCGAGCCCTTTCAAGTGCATCA GAGGATATTTGGAGGACAACCTGCAAAGATTGAAAATTTTCCCTGGCAAGTCTTCTTTAATCACCCCAGA GCTAGTGGGGCTCTTATTAATGAGTACTGGGTGCTGACGGCTGCTCACGTTTTGGAGAAAATCTCCGACC CTTTAATGTATGTCGGGACCATGTCTGTGAGGACAACTCTCTTGGAAAATGCCCAGAGACTCTACAGTAA ACGTGTCTTTATTCATCCCAGCTGGAAGAAAGAAGATGACCCAAACACACGGACAAATTTTGACAATGAC ATTGCCCTGGTGCAGCTGAAAGACCCTGTGAAAATGGGACCCAAAGTCTCCCCCATCTGCCTACCAGGCA CCTCCTCAGAGTACAACGTCTCACCGGGTGACATGGGGCTGATCTCAGGGTGGGGCAGTACAGAAAAGAA AGTCTTTGTTATCAACCTCAGAGGGGCAAAGGTACCAGTAACCTCTTTAGAAACCTGCAAACAGGTGAAA GAGGAGAACCCCACTGTAAGGCCTGAGGACTATGTTTTCACTGACAACATGATCTGTGCTGGCGAGAAAG GTGTTGACAGCTGTCACGGGGACAGCGGCGGGGCTTTTGCTTTCCAGGTCCCCAATGTCACGGTCCCCAA ATTCTATGTAGCTGGCTTGGTGTCTTGGGGGAAAAGGTGTGGGACTTATGGGGTTTACACAAAGGTAAAG AATTACGTGGACTGGATCCTGAAAACTATGCAGGAGAATAGTGGGCCCAGGAAGGACTAATCCGTAGGGC GACAACTCCCCTCAAGGGCATAGCAAGCTCACTGTTCTCAGATCCTATGGTGGTCCCCTTATTTCACAAC GATGGAGAGAAGATGTGGGAGTATGGTTAATGCTGAACTTGATTGTCAAAGAGTCTGCATGGAGGCAGGG TTGATCACTGAGCCGTGTTGGTTATTCAGTTACTATTGCTAACAACATGGCAGAAACCCTTCTATCTTGC GCTATTCCACAGGGATATCTTATCCGATCTTCCCTCATTTAACCTGCTTGAAATTCCTGAATAATTTACA GCAAAGCATGCTTCCAACCTGGTTCTGGCTGTATGGAGAGCCACGAAGGAGAGAGAGGGAGATTTGGGGG CATAGCATCATGGAATTCAGGCATCAACAGCATGGAACTCTGCATCTCTAACAAGATGCAGACAGGGAAC ACAGCCTTTTATTCTTTCTAGTGAGACTTACCGAATACTCAGAAGTTAGAAATGACTACCTTAGTTTTTC TCTGTGAGTTGCTTCAATATATTTCCTAGAAATTAATTTTCTTGTAATCCTCCTTTGTATCATACAGTGT AATGACTTAGTAAAATAGAAATTGAACATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5097206 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5097206 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003047 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source