EMAGE mouse gene expression spatial database

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EMAGE:32120

Acsl1 acyl-CoA synthetase long-chain family member 1 ( MGI:102797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003044_01	euxassay_003044_02	euxassay_003044_03	euxassay_003044_04	euxassay_003044_05

euxassay_003044_06	euxassay_003044_07	euxassay_003044_08	euxassay_003044_09	euxassay_003044_10

euxassay_003044_11	euxassay_003044_12	euxassay_003044_13	euxassay_003044_14	euxassay_003044_15

euxassay_003044_16	euxassay_003044_17	euxassay_003044_18	euxassay_003044_19	euxassay_003044_20

euxassay_003044_21	euxassay_003044_22	euxassay_003044_23	euxassay_003044_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 18 19 moderate expression: see section 05 08 17

stomach

moderate

regional

moderate expression: see section 03 04 05 06

kidney calyx

moderate

regional

moderate expression: see section 09 10 18 19 20 weak expression: see section 21

tongue

weak

regional

weak expression: see section 11 12 13 14 15

rectum

moderate

regional

moderate expression: see section 13 14

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 18

nose

moderate

regional

moderate expression: see section 07 08 09 10 11 16 17 18 19 20

midgut

moderate

regional

moderate expression: see section 09 10 13 14 15 16 17 18 19 weak expression: see section 11 12

trachea

strong

regional

strong expression: see section 11 12 13 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 17 18 19 20 21 22

hindgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 weak expression: see section 12

epidermis

strong

regional

strong expression: see section 22 23 24 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

naris

moderate

regional

moderate expression: see section 11 12 13 15 16 17

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nasal septum

moderate

regional

moderate expression: see section 12 13 14 15 16 17

lung

strong

regional

strong expression: see section 09 10 15 16 17 18 moderate expression: see section 19 20 weak expression: see section 08

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4882
Entity Detected:	Acsl1, acyl-CoA synthetase long-chain family member 1 ( MGI:102797)
Sequence:	sense strand is shown >T4882 GTGACAGCCGCCTGTGGAGCAGCCCGGGCAGCGGAGGAGAATTCTGCATAGAGAACCACCAACCCAGAAC CATGGAAGTCCATGAATTGTTCCGGTATTTTCGAATGCCAGAGCTGATTGACATTCGGCAGTACGTGCGC ACCCTTCCAACCAACACCCTCATGGGGTTTGGGGCTTTTGCAGCGCTCACCACCTTCTGGTATGCCACCA GGCCTAAGGCCCTGAAGCCACCATGTGACCTCTCCATGCAGTCAGTGGAAATAGCGGGTACCACTGATGG TATTCGAAGATCAGCAGTCCTTGAAGATGACAAGCTCTTGGTGTACTACTACGACGATGTCAGAACCATG TACGATGGCTTCCAGAGGGGGATTCAGGTGTCAAATAATGGTCCTTGTTTAGGTTCTCGGAAGCCAAACC AGCCCTATGAGTGGATTTCCTACAAAGAGGTGGCAGAACTGGCTGAGTGCATAGGCTCCGGGCTGATCCA GAAGGGGTTCAAGCCTTGCTCCGAGCAGTTCATCGGCCTCTTCTCTCAAAACAGACCCGAGTGGGTGATC GTCGAGCAAGGATGCTTCTCTTACTCAATGGTGGTCGTCCCGCTCTATGACACCCTTGGAGCTGACGCCA TCACCTACATAGTGAACAAAGCTGAACTCTCTGTGATTTTTGCTGACAAGCCAGAAAAAGCCAAACTCTT ATTAGAAGGTGTAGAAAACAAGTTAACACCATGCCTTAAAATCATAGTCATCATGGACTCCTACGGCAGT GATCTGGTGGAACGAGGCAAGAAGTGTGGGGTGGAAATCATCAGCCTCAAAGCTCTGGAGGACCTTGGAA GAGTGAACAGAGTGAAGCCCAAGCCTCCAGAACCCGAAGATCTTGCGATAATTTGTTTCACAAGTGGAAC TACAGGCAACCCCAAAGGAGCAATGATCACTCACCAAAACATTATAAACGACTGCTCAGGTTTTATAAAA GCAACAGAGAGTGCATTCATCGCTTCCACAGATGATGTGCTGATATCTTTCTTGCCTCTCGCCCATATGT TTGAGACCGTTGTAGAGTGTGTAATGCTGTGTCATGGAGCTAAGATAGGATTTTTCCAAGGAGATATCAG GCTGCTTATGGACGACCTCAAGGTGCTTCAGCCCACCATCTTCCCTGTGGTTCCCAGGCTGCTGAACCGG ATGTTCGACAGAATTTTTGGACAAGCAAACACTTCCTTGAAGCGATGGCTGTTGGACTTTGCCTCCAAAA GGAAAGAGGCGGAGCTTCGCAGTGGCATCGTCAGAAACAACAGCCTGTGGGATAAACTCATCTTCCACAA GATACAGTCGAGCCTGGGTGGGAAAGTCCGGCTGATGATCACAGGAGCAGCCCCGGTGTCTGCCACAGTG CTGACGTTTCTGAGGACAGCGCTCGGCTGCCAGTTCTATGAAGGCTACGGACAGACCGAGTGCACTGCTG GTTGCTGCCTGAGCTTGCCCGGAGACTGGACGGCAGGCCATGTTGGAGCCCCCATGCCTTGCAATTATGT AAAGCTTGTGGATGTGGAAGAAATGAATTACCTGGCATCCAAGGGCGAGGGTGAGGTGTGTGTGAAAGGG GCAAATGTGTTCAAAGGCTACTTGAAAGACCCAGCAAGAACAGCTGAAGCCCTGGATAAAGATGGCTGGT TACACACGGGGGACATTGGAAAATGGCTGCCAAATGGCACCTTGAAGATTATCGACAGGAAAAAGCACAT ATTTAAACTAGCCCAAGGAGAGTACATAGCACCAGAAAAGATTGAAAATATCTACCTGCGGAGTGAAGCC GTGGCCCAGGTGTTTGTCCACGGAGAAAGCTTGCAGGCCTTTCTCATAGCAGTTGTGGTACCCGACGTTG AGAGCCTACCGTCCTGGGCACAGAAGAGAGGCTTACAAGGGTCCTTCGAAGAACTGTGCAGGAACAAGGA TATCAATAAAGCTATCCTGGACGACTTGTTGAAACTTGGGAAGGAAGCCGGTCTGAAGCCATTTGAACAG GTCAAAGGCATTGCTGTGCACCCGGAATTATTTTCTATTGACAACGGCCTTCTGACTCCAACACTGAAGG CGAAGAGGCCAGAGCTACGGAACTATTTCAGGTCGCAGATAGATGAACTGTACGCCACCATCAAGATCTA ACGTGAGGAAGGATACTTAGAAGAAATGGCGCATCTCCACAATCCTCCTCGTACCAATGGCCTTCGAGTT GGTAACTTTGCCTGCAGCGAGTGTGGGAAAGGAAATGCCCTGCCGCCGGACTTGTCCACGGGGTCTTACC ATAGGGATAGTCGAGGGCACGGAACACTGCCTTACTTACAGTCACCTGTGTTGCAGCCCATGATCCCGGG GACACACAATTTCCAAAACGAGCCTTAAACATTGTAAAGGGGAACCCATAAAAGTGCTAAGTTATTTAAG ACTTCTTCAACCAATAAGGTGGATGCTACAAGTTCTGTCTCCTGTTTTTCTAACTGAGGGGTTAGGACTT ATTCTTTCTGATATGTCTGCTGCTTGCTGCGCGTTTTGCAGCTGTCTGCTGCTCTGAAGAGCACCGTACA CTGGAGGAAAGCTGTCCCTTTAAGAACAACTGTCCAGGCTGAAGAAAGTCACAGTGGACCAGAGGTTTTC CTTTTGAACCCCTCCTCCCCCTTGCCCCTTTCCCTCACCACCTCACATACAGTACACTCACATGACCTTT CTGGTTTGTAAGGGTTCCACACGGTCCCTGTTTGCGCCTGCTGGAACATGAGGTTTTCAGTAAACTAAAG AGCAGACCCTCTTCAGAACATGTGGGTGTCCAGTCTCAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098645 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098645 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003044 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source