EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:32122

Enpp4 ectonucleotide pyrophosphatase/phosphodiesterase 4 ( MGI:2682634)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003017_01	euxassay_003017_02	euxassay_003017_03	euxassay_003017_04	euxassay_003017_05

euxassay_003017_06	euxassay_003017_07	euxassay_003017_08	euxassay_003017_09	euxassay_003017_10

euxassay_003017_11	euxassay_003017_12	euxassay_003017_13	euxassay_003017_14	euxassay_003017_15

euxassay_003017_16	euxassay_003017_17	euxassay_003017_18	euxassay_003017_19	euxassay_003017_20

euxassay_003017_21	euxassay_003017_22	euxassay_003017_23	euxassay_003017_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4840
Entity Detected:	Enpp4, ectonucleotide pyrophosphatase/phosphodiesterase 4 ( MGI:2682634)
Sequence:	sense strand is shown >T4840 CCACGCGTCCGGCTGAGTTCCGCGCGCCAGACCGAAGTGGCGGTGGAGGCGGGTGAGTCCCGGAGGGAAA TGCCACTCCCTTCCCCAGCTCGCCGAGGGCGGAGGGTAGAGACGGGGCCCTAGCTGCTGTGTGCAACATG TTCAATATGAAGATATTAGTAATTCCTTTGTTTTGGGGACTTGTCACTGGCTATAAAGGGAACTCTTCCG ATAGCTCAGCACCTCGGTTACTTCTGGTATCCTTTGACGGCTTTAGAGCTGACTACCTGAAGAGCTATGA CCTTCCTCACCTCCAGAACTTTATCAAAGAAGGTGTCTTGGTGGAACATGTTAAAAATGTTTTTATCACC AAAACTTTTCCAAACCACTACAGCATTGTGACGGGCTTATATGAGGAAAACCATGGCATCGTGGCCAACT CCATGTATGACAGTGTCACAAAGAAACATTTTTCTGAATCCAATGATAAGGATCCGTTTTGGTGGAATGG AGCGGAGCCTATTTGGGTGACCAATCAGCTTCAGGAAAACAGGTCAAGCGCTGCCGCTATGTGGCCTGGC ACTGATGTGCCCATTCACAATATCACAGCTTCCTATTTTATGAACTATAGCAGCTCCGTGTCCTTTAAGG AAAGACTGGGTAACGTTACCACATGGCTCAGCAGTTCCAACCCACCAGTCACCTTTGCAGCGCTCTATTG GGAAGAACCAGATGTAAGCGGCCACAAGTATGGGCCTGAAGATAAAGAAAACATGCGCCGGGTATTGAAA GAAGTAGATGACCTTATTGGTGATATTGTCCTAAAGCTCAAGGTGTTAGGACTATGGGACAGCCTTAATG TGATCATTACAAGCGATCATGGGATGGCTCAGTGTTCTAAGAACAGACTGATAGATTTGGATTCCTGCAT CGACCGTTCAAACTACAGTGTTATAGACTTGACCCCTGTGGCTGCGATTCTTCCCAAAATAAATGTAACA GAGGTTTATGACAAACTGAAACGCTGTAACCCTCACATGAATGTTTATCTCAAAGAAGCTATCCCTAACA GATTTTACTACCAACACAGCAGTCGAATTCAGCCCATTATTTTGGTTGCTGAGGAAGGCTGGACAATCAC CCTGAACAAGTCATCATTTAAATTAGGTGACCATGGCTATGATAATTCCCTGCCTAGTATGCACCCGTTT CTTGCTGCCCACGGACCTGCCTTTAGAAAAGGTTACAGGCAGAGCACCATCAACACTGTGGATATTTATC CAATGATGTGCCACATCCTGGGACTAAAACCACATCCCAATAATGGAACCCTCAGTCATACCAAGTGCTT GTTGGTGGACCAGTGGTGCATTAATCTCCCAGAAGCCATTGGAATTGTTGTTAGTGCACTATTGGTATTA ACCATGCTAACAGGCCTCATGATATTCATGCGGAGCAGAGCATCCACATCCCGTCCATTCTCCCGTCTTC AGCTGCAGGAAGATGACGATGACCCTTTAATTGATTAATTCACCATGTCTTATTAATTTCAAAACTAAGG ATCCATGCAAGGAGTGGTGTTACAGCCATAAAAAAGGTATATTTCAAAAGACAAGACACACATGGCAAAA TTACTCTGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGCCCTGTTTTGGTGTGTTGCCTGATGCAAATAAC CCTGATGGTATTTAATTGCTGGTAAATCAGTTCAAACCACTAATGTCCCTAGCAAGACTAGCCTTTGAGA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065563 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065563 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003017 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source