EMAGE mouse gene expression spatial database

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EMAGE:32143

Acp1 acid phosphatase 1, soluble ( MGI:87881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003011_01	euxassay_003011_02	euxassay_003011_03	euxassay_003011_04	euxassay_003011_05

euxassay_003011_06	euxassay_003011_07	euxassay_003011_08	euxassay_003011_09	euxassay_003011_10

euxassay_003011_11	euxassay_003011_12	euxassay_003011_13	euxassay_003011_14	euxassay_003011_15

euxassay_003011_16	euxassay_003011_17	euxassay_003011_18	euxassay_003011_19	euxassay_003011_20

euxassay_003011_21	euxassay_003011_22	euxassay_003011_23	euxassay_003011_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19 20

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 06 07 22 weak expression: see section 23

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 16 17

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4650
Entity Detected:	Acp1, acid phosphatase 1, soluble ( MGI:87881)
Sequence:	sense strand is shown >T4650 GGGCGCCAGGGTCTGCACCGAAACATGGCAGAGGTTGGGTCCAAGTCAGTGCTGTTCGTGTGTCTCGGTA ACATTTGCCGGTCACCCATTGCAGAAGCAGTATTCAGGAAACTGGTAACTGATGAAAAGGTTTCAGATAA TTGGAGGATAGACAGTGCGGCTACATCCACCTATGAAGTGGGGAACCCTCCTGACTATCGAGGGCAGAAC TGCATGAGAAAACATGGCATCCACATGCAGCACATTGCACGGCAGATTACAAAAGAAGACTTTGCCACAT TCGATTATATACTATGTATGGATGAAAGCAATCTGAGAGATCTCAATAGAAAAAGTAATCAAGTTAAAAA CTGCAAAGCTAAAATTGAGCTACTTGGGAGCTATGATCCACAGAAACAGCTCATCATTGAAGATCCCTAT TATGGCAATGACTCTGACTTCGAGGTGGTGTACCAGCAATGCCTTAGGTGCTGCAAGGCCTTCCTGGAGA AGACTTACTAGCTGGTCCTAAGCCCCACCATTGAGCAGCTCACTCATCAGTGCTGTGCCCAAGGGTGGTG GCAGTCCTTAGCCCCATACCCCATCTCTCTTTTCAGCTAACTTACTGTGTATCTTTAAAATAATTGTAGG TGGGAATTAGGCATATGTTCAGAAGGATAAAAGCATTTGAGTCAGACAGTTTGAGGTGTGGCTAAGCATT CTTAGACTAACTAAACCTCTGACCTTGCGGTGATTACAAAACAGTGGAACAAGCAAATATGGAACAAAAA AACAAAAACAAAAACAAAAACCCAGTAAAGTAAGAGTGACCTAGAAGGTCCAGATCAGCCTCTGAGCTCG GCAAGCCTGGGTCGTCTGGTCTAAGTGGAGTGTGTGCATGACCCACACCCAGTGTGCTGTTTGCTTGCCG TGTACTCTCTACTCTAGGCTCTCGTTGTGACAATAGCTCCATTCACGGCAGCCTTCCAGTTAACGCTGGC AGTTTAAGCTCAGACACACTGAGGGTGTTGAGGGATTTGAGAGAGGACACGCTGGATGTGCTGATGGGCC TGGGACTCCAGCAGGCCGTCCTGGGCTGGACAGTGACACCCTGTCTAAAAGGGGAACAAAAATAACAACG GATGGGAGCAGCAGATGGATAAAGTTACCATCTTCAGTCTTACTTGGTTTTTGTCTACTTTCCTGAGCTT TGTTCTTGTTTGAGCCACTTTGCTTTAAAAAAAAAGTAAGTGTGCTTAAATACTGGTATGTGTTTGTAAG TGTTCTCTCATCGGCAATTTACAAAGTTATAGGCAAGCATGAGTAATTTTTGTGTATTTTCAGAAAAGAG ACCTCAAATTTATATGAATGTATGTCAGAAAGGAACATGAATTCAACTGGGAAGTTATCAAATACAACTG AGATACAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5010346 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010346 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003011 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source