EMAGE mouse gene expression spatial database

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EMAGE:32144

Polr3e polymerase (RNA) III (DNA directed) polypeptide E ( MGI:1349452)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003027_11	euxassay_003027_12	euxassay_003027_13	euxassay_003027_14	euxassay_003027_15

euxassay_003027_16	euxassay_003027_17	euxassay_003027_18	euxassay_003027_19	euxassay_003027_20

euxassay_003027_21	euxassay_003027_22	euxassay_003027_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4649
Entity Detected:	Polr3e, polymerase (RNA) III (DNA directed) polypeptide E ( MGI:1349452)
Sequence:	sense strand is shown >T4649 CCACGCGTCCGCCCACGCGTCCGGGCCGCTGCCTGATAGAAGACCCGGCCGCCGCCGTCTCTGCAGCCCG CGGGTCCCTGGGCCGTTCCGCCGCCCGCGCGCGGCCCCGGCGGAGAGATCGAGTTGAGGACTGTGAGGTG GGCTCCCTAGTATGGCCAATGAAGAAGATGACCCAGTCATACAGGAGATCGATGTGTACTTGGCTAAAAG TCTGGCAGAGAAGCTGTATCTCTTTCAGTATCCTGTGCGTCCTGCTTCAATGACCTATGATGACATTCCA CATCTCTCAGCCAAGATCAAGCCCAAACAGCAGAAGGTAGAGCTTGAGATGGCCATCGACACCCTGAATC CCAACTACTGCCGCAGCAAAGGGGAGCAGATCGCACTGAACGTGGACGGTGCCTGTGCTGACGAGACTAG CACATATTCCTCAAAGCTGATGGACAAGCAGACCTTCTGCTCCTCCCAGACCACCAGCAACACAGCTCGT TATGCCGCTGCACTCTACAGGCAAGGTGAGCTCCACCTGACACCTTTACATGGCATCCTACAGCTTCGGC CCAGCTTCTCCTACCTTGATAAGGCAGATGCCAAGCACCGAGAGAGGGAGGCAGCCAATGAGGCAGGGGA TTCTTCTCAGGACGAGGCAGAAGAAGACGTTAAGCAGATCACGGTACGGTTCTCCAGGCCAGAGTCCGAG CAGGCTCGCCAGCGCCGCGTGCAGTCCTATGAGTTCTTGCAGAAGAAGCATGCAGAAGAACCCTGGGTCC ACCTGCACTACTATGGCATGAGGGACAGCCGCTCAGAGCATGAGCGCCAGTACCTGCTGTGTCAGGGATC CAGCGGGGTAGAGAACACGGAGCTCGTCAAGTCACCCAGTGAATACCTCATGATGCTCATGCCGCCCAGC CCAGAGGAGGAGAAGGACAAGCCTGTGGCTCCCAGCAATGTCTTATCTATGGCCCAGCTGCGTACATTGC CTCTGGCTGATCAGATTAAGGTCCTGATGAAGAACGTGAAAGTCATGCCCTTCGCTAATCTCATGAGCCT CCTGGGCCCCTCTGTGGACTCTGTGGCTGTTCTACGTGGCATCCAGAAGGTGGCGATGTTAGTCCAAGGA AACTGGGTGGTAAAGAGTGACATCCTGTATCCCAAGGATTCTTCCAGCCCTCACAGTGGCATGCCTGCTG AGGTGCTCTGCAGGGGCCGAGACTTTGTTATGTGGAAGTTCACACAGAGCCGATGGGTGGTAAGAAAGGA GGTGGCAGCAGTGACCAAACTCTGCGCTGAGGATGTGAAGGACTTTCTGGAGCACATGGCTGTAGTGAGA ATCAACAAAGGCTGGGAGTTCTTACTGCCTTACGACCTGGAGTTTATCAAGAAGCATCCAGATGTGGTCC AGCGGCAGCACATGCTGTGGTCGGGCATCCAGGCCAAATTAGAAAAAGTCTATAATCTCGTAAAAGAAAC CATGCCAAAGAAGCCGGATGGACAATCAGCGCCTGTTGGTCTGGTCTCTGGGGAACAGCGGGTCCAAACA GCCAAAACCAAGGCCCAGCAGAACCATGCCTTCTTGGAGCGTGAACTGCAGCGACGGAAGGAGCAGATGC GGGCAGCCACAGTCCTGCCCAGCGTGCAGATCAAGGAGGAACCCCTGAGTGAGGAGGAAGCCGATGGGGC TGAGCTGGAGGCTGAGGAAGAGGAGCCCATGGACACTGCACCCAGCACTTGCCTCAGCACCAAGTTGGCC AATGGGCTGCCTGCTGGGCGGGCAGTGGGTGGGGACAGCCTAAATGGACACCCAGTTCCAGGCTGTGCCA GCAACCCGGTTGCCTGTGAACTGAAGGCTTTTGTGGAAGCCACCTTTCAGAGACAGTTTGTGCTCACGCT GAGCGAACTCAAGCGCCTCTTCAACCTGCACCTGGCAGGCCTGCCCCCGGGCCATATACTCTTCAGTGGC GTCTCAGACCGAATGCTACAGGATACGGTGCTGGCCGCTGGCTGCAAGCAGATACTGGTGCCTTTTCCCC CACAGACTGCTGCTTCCCCTGATGAGCAGAAGGTATTTGCCCTCTGGGAGTCTGGAGACATGAGTGACCA GCATCGGCAGGTTTTGCTTGAAATATTTTCCAAAAATTACCGAGTACGTCGGAACTTGATCCAGTCTCGG CTGACTCAAGAATGTGGAGAAGAGCTAAGCAAACAGGAGGTGGATAAAGTGCTAAAGGACTGTTGTGTAA GCTGTGGTGGCATGTGGTACCTTAAGGGGACAGTACAATCCTGACAATAGGAGCAAACTGCTAACTCGAC GAATCCAAGCCCAGGGAAGGATGGCAGAGCCACACGTAGTCTTGACTTGCCTCAAGACACAGGAAGAGGA TCTGCCTACCTGGTGGCCTGTGGCGCTGCCTGGCCCAGAGGACAGAGGGGCTCATGCTCAGCCAGTGGCA GGGTCAGCGTAAGTGAGAGCGCTCCCAGCAGAGACCAGCGGGCCTTCTTTGCAGTACAATTTGAAATTCC TGATGTATTTTGCTTATTGGGTTTCATTCTCATAATAAAGAGAGTGTACACTGACATGGGCAGGATTACG AAAATCATGGTTTAATATTTTCCTCTTTTAAATGAATGCCAACATAGTTAAAGATATGTTTACAAGATGA AGAGAACCTTAAGGTTTTTAATATTTAAAATGCCTAGAAGCCAATAATTAGTCTTAGAGTTTCTCTCTGC TAAGACTCCTGCCAATTTTATTAAACCAAATTGAACATTTTACTGTTGGGTTTCTGAGGCCACTTTACCT TTATAGCAGCCTGCTTTCTGTAGCGGTCTCAACTGTTTACACAAACCATAGCAAAACATTAGATTAACTC CATCTTCTCCTAATGTTTGCATAAAGGTGCAAACTAAACCAGGTAAGTGTGGAACAGTATATAAGCGAGG TTATCACTTTGATGTAAAAAAATCTATTTTGTGTATTTTTAAAATAAATGCTAACACTAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5010681 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010681 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003027 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source