EMAGE mouse gene expression spatial database

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EMAGE:32147

Limk2 LIM motif-containing protein kinase 2 ( MGI:1197517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004119_01	euxassay_004119_02	euxassay_004119_03	euxassay_004119_04	euxassay_004119_05

euxassay_004119_06	euxassay_004119_07	euxassay_004119_08	euxassay_004119_09	euxassay_004119_10

euxassay_004119_11	euxassay_004119_12	euxassay_004119_13	euxassay_004119_14	euxassay_004119_15

euxassay_004119_16	euxassay_004119_17	euxassay_004119_18	euxassay_004119_19	euxassay_004119_20

euxassay_004119_21	euxassay_004119_22	euxassay_004119_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19

tongue epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 17 18

stomach

moderate

regional

moderate expression: see section 03 04 05 06

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 17 18

oral cavity

moderate

regional

moderate expression: see section 13

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 10 11 12 13 15 16 17

lower jaw molar

weak

regional

weak expression: see section 05

vagus x ganglion

weak

regional

weak expression: see section 07

upper jaw molar

weak

regional

weak expression: see section 05

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 17 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

naris

moderate

regional

moderate expression: see section 12 13

esophagus epithelium

moderate

regional

moderate expression: see section 11 12

pharynx epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14

lower jaw incisor

weak

regional

weak expression: see section 07 11 12

upper jaw incisor

weak

regional

weak expression: see section 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6160
Entity Detected:	Limk2, LIM motif-containing protein kinase 2 ( MGI:1197517)
Sequence:	sense strand is shown >T6160 CCCACGCGTCCGCCCACGCGTCCGAGGGGCAGAGACAGCCTCTTCTCTAGACCGCAGGCGGGCGCGGACC TGAGGCGCTGCTGCCGGGTGGTCCTTCCTGTGTTGTCCGCGCCCTGGACCATGGCGGCGCTGGCGGGTGA TGAAGCCTGGAGGTGTCGGGGCTGTGGCACCTATGTTCCCCTGAGCCAGAGGCTCTATAGGACTGCCAAT GAAGCCTGGCATGGCTCCTGCTTCCGGTGTTCCGAATGCCAGGAATCCCTCACCAACTGGTACTATGAGA AGGATGGGAAGCTGTACTGCCACAAGGACTACTGGGCCAAGTTTGGAGAATTCTGCCATGGGTGCTCCCT GCTGATGACGGGGCCAGCCATGGTGGCTGGGGAGTTTAAGTACCACCCAGAGTGCTTTGCCTGCATGAGT TGCAAGGTGATCATTGAGGATGGAGATGCATATGCCCTGGTGCAGCACGCTACACTCTACTGTGGGAAGT GCCACAACGAGGTGGTGCTGGCACCCATGTTTGAAAGACTGTCTACAGAGTCTGTCCAGGATCAGCTGCC CTACTCTGTCACGCTCATCTCCATGCCTGCTACCACCGAGTGTAGACGCGGCTTCTCGGTGACTGTGGAA AGTGCCTCTTCTAACTATGCCACCACCGTTCAAGTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACA ACCGAAATGCCATCCACCCTGGGGACCGCATCTTGGAGATCAATGGGACCCCTGTCCGTACTCTGCGAGT AGAGGAGGTGGAGGATGCAATAAAGCAGACAAGCCAGACACTTCAGCTGCTGATTGAACATGACCCTGTG CCCCAGCGCCTGGACCAGCTACGGCTAGATGCCCGACTTCCTCCCCACATGCAGAGCACCGGACACACGC TCATGCTCAGCACCTTGGACACCAAGGAGAATCAGGAGGGGACACTGAGGAGACGTTCTCTGAGGCGCAG TAACAGCATCTCCAAGTCTCCGGGCCCCAGCTCCCCCAAGGAGCCCCTGCTCCTCAGCCGGGACATAAGC CGCTCAGAATCCCTCCGCTGCTCTAGCAGCTACTCCCAGCAGATCTTCCGGCCCTGCGACCTGATCCACG GGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCCATCAAGGTGACTCACAAAGCCACAGGCAAAGTGAT GGTCATGAAGGAGTTGATCCGCTGTGATGAGGAAACCCAGAAGACTTTCCTGACTGAGGTGAAAGTGATG CGGAGCCTGGACCACCCTAATGTGCTCAAGTTCATCGGAGTTCTGTACAAGGACAAGAAGCTGAATCTGC TGACCGAGTACATCGAGGGAGGCACACTCAAAGACTTCCTGCGCAGTGTGGACCCGTTCCCCTGGCAACA GAAGGTCAGGTTTGCCAAAGGCATCTCCTCTGGAATGGCCTATTTACACTCCATGTGCATCATCCACCGG GACCTGAACTCACACAACTGTCTCATCAAATTGGACAAGACAGTGGTAGTAGCCGACTTTGGGCTGTCAC GGCTTATAGTAGAAGAGAGGAAAAGGCCTCCAGTAGAGAAGGCCACCACCAAGAAACGCACCTTACGCAA GAGTGACCGCAAAAAGCGCTACACTGTGGTGGGAAACCCCTACTGGATGGCCCCCGAGATGCTGAATGGC AAGAGCTACGATGAGACGGTGGATGTCTTCTCTTTTGGGATCGTTCTCTGTGAGATCATTGGGCAGGTAT ATGCGGATCCTGATTGCCTGCCCCGCACACTGGACTTTGGCCTCAATGTTAAGCTTTTCTGGGAGAAGTT TGTCCCAACAGACTGTCCCCCAGCCTTCTTCCCCCTGGCTGCTATCTGCTGCAAACTAGAGCCTGAAAGC AGACCGGCATTCTCAAAGCTGGAGGACTCGTTCGAGGCGCTCTCCCTGTTCTTGGGGGAGCTGGCCATCC CACTGCCAGCAGAGCTGGAAGACCTGGACCACACTGTGAGCATGGAGTATGGCCTAACCCGGGACTCGCC ACCCTAGCCCTGGTCCCTCCCCCTGCAGGGGGACATTCCACAGCCAGCATTGCCCCCTCTGCACGCTGTC CTTGCTGTGAGCAGGACTGCCTAGGTTTCCTGTGGATTGAAGGCTTGCTTAGAGGCACAACAAGCCATCC CTACTACCTCCCCAGGAGGCACTTGGGTGCAGGGAAAAAAATTCTCCGCAAGTTTTGGGGGCCTAGTTTC TATCTGTAAATCCAACACTCACCTGAAAGCTGTGAAGAGGATAAAAGAGCCTGGATTATAGACCAAGAGT CTGTTACTCGGGACTACTTGGGACCTCTGTGGATTGAGATTCTGGGAGGCTGTAGCTTTACACTAATCAA CATGATCTGGGCCTGCTGGGCAAGATGACGGAGTGAACTGGCCTGAGGGCTCTGGAGTCTGGCTCAGCAA GGTGCAGGACTTGAAGCGGTAGTATGAGTTACAGAAACGGGGAACCTTCAAAGCATGTGAAATGACAGTA GCGATTTCTCCTCCATCCCAGGCCATGCCCTTTGGAGCAGGTTTTAAAGAGTCTTAGTATGTGGTAAAAC CTACAGAATCCAACAAAGGACTGGTCTCTGCTCACAGCCCAGGGACCACATCAGTGCATGGGGAGTTTCC ACTTCAGCATCCTGTCTCAACAAGCAGTCACTCACAAAAACACCAAAGAAACTGGGGCTGCAAACAGGCC CTGCCCTCTAAAAGCCCTGTATCCAGGCTGGTGGGCTCAGTCAAAAGCTCAGCATGTTGCCTAACAGTAC ATGGGTTCTGGATGCCAGCCTGGTTTGCCTCAGACTTTGGGTCTAAGCAGAGAGAAGGGGGTCCCATCAA TTTCTTTGCACCCTTGTGGCAGCTACTGCACATCCTCTAGAATGTCTGGGCCAGGCAGCAATGTGGGACA GGCATTGGTGGCTGCTGAGTCCCCAAACTGAGATAGGAAAAGAACACCCTGAGCTGGGCCTTGTAGATTC TACCTCTTTTTGTTGCTGGTCCAGCTTGCTTCCTGAGCCACACTCGAGGGCCATGGGCATGGGCAACTAT GGGGGCACTTTCTACCCCTGGCTGCCAGGTGTCCAGGGACTCTGTAGCTCACCAAGGTTTGTCATCTAAG CGGGAGCTCACGCCATGTTTAACAAATTGGGAATGGGTTTGGTTATAAATACATGTGATGGGTTGTACTG GTGGGGGGATTCAGGAAGAGTGGTTGCTGTTATTAGCTTTTATCTCTGGGGTGATATGTGAGATATTTTG TACATAGACGAAATGAATTATGGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496836 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496836 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source