EMAGE mouse gene expression spatial database

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EMAGE:32149

Six3os1 Six3 opposite strand transcript 1 ( MGI:1925118)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_014141_01	euxassay_014141_02	euxassay_014141_03	euxassay_014141_04	euxassay_014141_05

euxassay_014141_06	euxassay_014141_07	euxassay_014141_08	euxassay_014141_09	euxassay_014141_10

euxassay_014141_11	euxassay_014141_12	euxassay_014141_13	euxassay_014141_14	euxassay_014141_15

euxassay_014141_16	euxassay_014141_17	euxassay_014141_18	euxassay_014141_19	euxassay_014141_20

euxassay_014141_21	euxassay_014141_22	euxassay_014141_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 14 15 16 17

telencephalon mantle layer

strong

regional

strong expression: see section 07 19 20 21 moderate expression: see section 10 18 22

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 15 16 17 18

pituitary gland

strong

regional

strong expression: see section 09 10 11 12 13

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 14 15 16

anterior naris epithelium

strong

regional

strong expression: see section 10 11 moderate expression: see section 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

external naris epithelium

strong

regional

strong expression: see section 10 11 moderate expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40369
Entity Detected:	Six3os1, Six3 opposite strand transcript 1 ( MGI:1925118)
Sequence:	sense strand is shown >T40369 CTTTGAGTGACCCTCATTAGCCTGGCGCCCTGCTAAGGCCCCACCATTGCAAAAAAGCTGCTGGGCCCGG CATTTGTACGCCTTGTTAGCGCTGCTTAATGAAGCCGCCCCGACGGCTCAAAGGCGGCCTCTGGGGCCGG CAGCGCGCACCTCTCGGCACCCCGCTCTGCCTAATGAGCCCCGCCGCCCGCTAAAGGCCCTTCCACCCCC CACATCCTCAAACAAGAGGGGTTTGGCTCCGTCTAGGACCTGAACACCTCCCACCACCGGGCCCAGGAGG GTGGGCACAAACCCAGTCTAGCTGGCTACACGGTGATCTAGCCCCTGGAGAAAGAAGCCAGCTACTTGGA GTATGCAAGCTAAAAATGGTTGTGTGAGTGGGAAAGTAGGGAAGTGGATTTTACAGCCACTAGGTCTAGC TCACCCGTGGGTACCACGAACTTTCCCCCACAGGGCCAGCTGTAAATCAATTAACCAGCCCAGCTCCTTA AAGGTGCATCTGGACTAGTGTTAGAACGTCAGAAGGGCTGGTACACAGGGGAGGGTCAGCTTTCCACTCC CCCCTCAAATTCCTTTGCTCTGGTCTCCCCAGGAGGCCTGGCACTTCTCCCCAGGTGGCCTCACACTTTC TGGAAGGTCAACCTTCCAGGCAACTAGAACTCTAGGCCTGTATTTGAGAAAGGATGGGCAGGTTCAGTTC ACACCCCTCGGAGAATGACTTCCAGTTTCACACAATCCCATCCCTGGTCTCTCTTCCATT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93584. Forward Primer - name:093584_F_cDNA_E130112H22Rik, sequence:CTTTGAGTGACCCTCATTAGCC; Reverse Primer - name:093584_N_SP6_cDNA_E130112H22Rik, sequence:AATGGAAGAGAGACCAGGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_014141 same experiment
	EMAGE:29902 same embryo
	EMAGE:29872 same embryo
	EMAGE:29875 same embryo
	EMAGE:30346 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source