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EMAGE:32151

Arhgap26 ( MGI:1918552)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_016565_01 euxassay_016565_02 euxassay_016565_03 euxassay_016565_04 euxassay_016565_05
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euxassay_016565_11 euxassay_016565_12 euxassay_016565_13 euxassay_016565_14 euxassay_016565_15
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euxassay_016565_16 euxassay_016565_17 euxassay_016565_18 euxassay_016565_19 euxassay_016565_20
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hypothalamus mantle layer
weak weak
regionalweak expression: see section 09 10 12 13
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 11 16 17 18
telencephalon mantle layer
strong strong
regionalstrong expression: see section 20 moderate expression: see section 05 19 21 weak expression: see section 06 18
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 13 14
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38831
Entity Detected:Arhgap26, ( MGI:1918552)
Sequence:sense strand is shown

>T38831
GGACAGTAGCAATCACACCAAAAGCTGCTTCAGTCATAGAACTTCCAGAAGCATCCATTATTGAGGCAGG
GGGAAGTAGACTAAAGAGGGTGAGATCATCTGTTCTTGGCCGGCTATATCTGCTTCCTGTGACAAGGCTG
TTTACCTAGCTTCATAGCCAGCGAGCTTGGGGGTGCACCAGTGACCACACATTTGCCCTGGCACCCACTG
TTCTTAGTAGACAAGGGAACCAAGCGTCTGCTTAGCTACCTGTCTTTCAAAGGAATGTTCTGGCTGTCTA
GGAAGGGAGTTGTCTGCCAATTCTGTCAAACTTGACAGTGATCACTTACGTTGATAGCCCTGTCCCAACA
GCCCTTTAATCAGTCAGGTGCCCCTCACTCCCCATAGTAGTGCATGAAAAAATATCTTTTGTATTCAAAT
ATTTCTGCACCCATGCAGATCTAGTGTTTGTCTGTGAATGTAGTATCAAAATGACAACAGCAGAGAGCTG
GGACTGAACCTTGGACATGCCTCCAACATGCTGAGACAAGACTCCACCAGGCGATGACGGGCCTTCTTCC
CTTTGCCGCCCACAGCACGTGTCTAACTTAAAGAGCCACCCTAAGTAGACAGAGATTCCAGACGTCACTC
GGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236607. Forward Primer - name:165762_F_cDNA_4933432P15Rik, sequence:GGACAGTAGCAATCACACCAAA; Reverse Primer - name:165762_N_SP6_cDNA_4933432P15Rik, sequence:CTCCGAGTGACGTCTGGAAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_016565 same experiment
 EMAGE:30726 same embryo
 EMAGE:30715 same embryo
 EMAGE:30714 same embryo
 EMAGE:30729 same embryo
 EMAGE:30727 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS