EMAGE mouse gene expression spatial database

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EMAGE:32155

Hkdc1 hexokinase domain containing 1 ( MGI:2384910)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003023_01	euxassay_003023_02	euxassay_003023_03	euxassay_003023_04	euxassay_003023_05

euxassay_003023_06	euxassay_003023_07	euxassay_003023_08	euxassay_003023_09	euxassay_003023_10

euxassay_003023_11	euxassay_003023_12	euxassay_003023_13	euxassay_003023_14	euxassay_003023_15

euxassay_003023_16	euxassay_003023_17	euxassay_003023_18	euxassay_003023_19	euxassay_003023_20

euxassay_003023_21	euxassay_003023_22	euxassay_003023_23	euxassay_003023_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

moderate

single cell

moderate expression: see section 01 02 03 04 05

ventral grey horn

strong

regional

strong expression: see section 14 15 16 17

stomach

strong

regional

strong expression: see section 05 06 07 08 09 10 11 moderate expression: see section 03 04 12

kidney calyx

strong

regional

strong expression: see section 09 10 11 12 13 21 22 23 24 moderate expression: see section 20

pons ventricular layer

strong

regional

strong expression: see section 14

4th ventricle lateral recess

strong

regional

strong expression: see section 20 21 22 23 moderate expression: see section 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 24

midgut

strong

regional

strong expression: see section 09 10 11 13 17 18 19 moderate expression: see section 12 14 15 16 20 21 22 23

pancreas

strong

regional

strong expression: see section 11 12 13 14 15 18 moderate expression: see section 19 20

not examined

regional

not examined expression: see section 02 03 04 05

midbrain ventricular layer

strong

regional

strong expression: see section 14

hindgut

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 16 20

liver lobe

strong

regional

strong expression: see section 05 06 07 08 09 10 11 moderate expression: see section 01 02 03 04 12 13 14 15 16 17 18 19 20 21 22 23 24

lateral ventricle choroid plexus

strong

regional

strong expression: see section 20 21 22 moderate expression: see section 07 08 09 10 11 12 13 15 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4273
Entity Detected:	Hkdc1, hexokinase domain containing 1 ( MGI:2384910)
Sequence:	sense strand is shown >T4273 CACCCACCTCCCCGCCCAAACTAGAGGCGTGCAGCACTCCGCAGACAGAGAGTGTACTGAAGACTCGGCG CTCTCGGGAACAACCGCACTTGGGAACATGTTTGCAGTACACTTGGTGGCGTTTTACTTCACCAAGCTGA AGGAGGACCAGATCAAGAAGGTGGACAGGTTCCTGTATCACATGCGGCTCTCGGATGAGACCCTGGTGGA CATCATGGCCCGCTTCCAGGCCGAGATGGAGAAAGGCTTGGGGAAGGACACCAACCCCACTGCCTCTGTG AAGATGCTGCCCACATTCGTCAGGGCCATTCCAGATGGCTCAGAAAACGGGGAATTCCTTTCCCTGGATC TCGGAGGATCCAAGTTTCGGGTCTTGAAGGTGCAAGTCTCCCAAGAGGGCCAGCAAAATGTTCAGATGGA GAGCCAGTTCTACCCAATGCCCAATGAAATCACCCGTGGGAATGGCACGGAGCTTTTTGACTACGTAGCC GACTGCCTGGCAGATTTCATGAAGACCAAAAATCTGACACATAAGAAGCTGCCTCTGGGCTTCACCTTTT CTTTTCCCTGCAGACAGAATAAGTTGGAGGAGGGTGTTCTGCTTTCGTGGACCAAGAAGTTCAAGGCCCG GGGAGTTCAGGACACAGACGTGGTGAACCGCCTGGCCACTGCCATGAAGAAGCACAAGGACCTGGACGTG GACATCCTGGCTTTGGTTAACGACACTGTGGGGACCATGATGACTTGTGCTTATGATGATCCCAACTGTG AAGTCGGCGTTATCATCGGAACAGGCACCAACGCGTGTTACATGGAGGACATGAGCAACATCGACCTCGT GGAAGGGGACGAGGGAAGGATGTGTATCAACACGGAGTGGGGGGCCTTTGGGGACGATGGGGCTTTGGAG GACATCCGCACCGAGTTTGACAGAGAGCTGGACCTGGGCTCGCTCAACCCCGGGAAGCAGCTGTTCGAGA AGATGATCAGCGGGCTGTACATGGGGGAGCTGGTCAGGCTTATCTTGCTGAAGATGGCCAAAGTTGGTCT CCTGTTTGGGGGTGCAAAATCCTCTGCTCTCCATACAAAGGGCAAGATCGAAACACAGCACGTGGCTGCC ATGGAGATGAGTAAAGAAGGCCTGGCCAACACTCGAGAGATCCTGGTGGATTTGGGCCTGGAGCCCTCTG AGTCAGACTGCATCGCTGTCCAGCACGTGTGCACCATCGTCTCTTTTCGCTCAGCCAATCTCTGCGCAGC GGCTCTGGCAACCATCCTGACTCGACTCCGAGAGAACAAGAAGCTGGCCCGGCTCCGCACCACAGTGGGC ATGGATGGAACTCTCTACAAAACACACCCTCAGTACCCCAAACGCCTACACAAGGTGGTGAGGAGACTGG TCCCAAACTGTGATGTCCGCTTCCTCCTGTCAGAGAGTGGAAGCACCAAGGGGGCCGCCATGGTGACCGC AGTGGCCTCTCGGGTACAGGCCCAGCGCAAGCAGATCGACAAGGTGCTGGCTTTGTTCCAGTTGACCCGA GAGCAACTCCTGGGTGTGCGGGACAAGATGCGGGCTGAACTAGAGTACGGGCTGAAGAAAAAGACCCACT CTCTGGCTACCGTGAAGATGCTTCCCACCTATGTCTATGGGATGCCGGATGGCACAGAGAAAGGAAAGTT CCTTGCCCTGGATTTGGGGGGAACCAACTTCCGAGTCCTCCTGGTAAAGATCAGGCGGAGGTCTGTGCGC ATGTACAACAAGATCTTCGCCATCCCTCTGGAGATCATGCAGGGCACTGGGGAGGAGCTCTTTGACCACA TCGTACAGTGCATCGCTGACTTCCTGGACTACATGGGCCTCAAGGGCGCCCAGCTGCCTTTGGGCTTCAC CTTCTCCTTCCCCTGCAGACAGACATGCATCGACAAGGGGACTCTAGTAGGGTGGACCAAAGGCTTCAAG GCCACCGACTGTGAAGGGGAGGACGTGGTGGACATGCTCAGAGAAGCCATCAAGAGGAGAAACGAGTTTG ACCTGGACATTGTTGCAATCGTGAACGACACAGTGGGGACCATGATGACCTGTGGCTATGAAGATCCCAG GTGTGAGATTGGCTTGATTGCAGGGACAGGAAGCAACGTGTGCTACATGGAGGAAATGAGGAACATCGAG CTGGTGGATGGGGACGAAGGCCGGATGTGCGTCAACACGGAGTGGGGCGGGTTTGGTGACAATGGCTGCA TCGATGACATCCGCACCCAGTATGACAAGGAGGTGGATGAGGGCTCCCTGAACGCCGGCAAGCAGAGATA CGAGAAAATGACCAGTGGAATGTACCTGGGGGAGATTGTGAGGCGGATCCTGATCGACCTCACCCGGCAG GGGCTCCTCTTCCGAGGGCAGATTTCAGAGCGACTCCGGACCAGGGGCATCTTTGAAACCAAATTCCTGT CCCAGATTGAAAGTGACCGGCTGGCCCTCCTGCAGGTCAGGAGGATCCTGCAGCAGCTGGGCCTGGACAG CACGTGCGAAGACAGTATTGTGGTGAAGGAGGTGTGTGGGGCTGTGTCTCGGAGGGCCGCCCAGATGTGT GGTGCCGGCATGGCTGCCATCGTGGAGAAGAGGAGAGAAGACCAAGGCCTCCAGCACTTCAAGGTCACCG TGGGCGTGGATGGCACACTGTACAAGCTGCACCCGCACTTCTCCCGGATACTGCAGGAGACGGTGAAAGA GCTGGCCCCTCAGTGTGATGTGACATTTATGTTGTCAGAAGACGGCAGCGGGAAGGGAGCAGCTCTGATC ACAGCTGTAGCCAAGAGGTTACAGCAGCCACGGAAGGACATCTAGGTGGCCCAGCCGGGTGATGACCAGG GACCACCAGACGACCTCCTGGCTCACCTTCCTCTCTCCGTACTCTAAGCATCCTGGTGCTTGCTTGGCAG TGACACTGGGTGGCACCTCCATTTGGCATATTTTAGCCAAAGATGGGCTGACTTTCAAAATCAAAGGATC TGGCCCCAAAGATCCCCCTTTTGGCAAGTCACTTGAGCTGTGAGCGTTCAGTAGTATCAGGTCTTGGGTT GCTGGAGTGTCCCTTTCAGGCCTTATGCTACCTCTGGTGGGAGATTGGGTCTGAACCCTTGAGAGGGAGA AAGGCTTAGTGAACTAGGGGATCTAGGTTAACATGGCCTCACTTGTCATGTTGACTTTATAGGCACCAGG GAAGCCAGGGCATTGGCCCCTGCATCTCTGAGTGTGGAGGAGCCCACTGCCCTGGAGCCAGGAGGAATCT CACCCACTCAGGGCGCTCTGTGAGCATCTGGGGTGTCACCCCCCCATGTTTGTATCATGGGGCGGGACAC CATCCCAGGTACTGTTTATCATTCATGTGACACAGTAGGTGTCGCTGTTTTCTGCATTTCTTTCAAGGAC TGTTAGGTATACAAATATTGTCCATGAATAAAAGAAACTTGAGTTTCCAGGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504302 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504302 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003023 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source