EMAGE mouse gene expression spatial database

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EMAGE:32157

Qdpr ( MGI:97836)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002173_01	euxassay_002173_02	euxassay_002173_03	euxassay_002173_04	euxassay_002173_05

euxassay_002173_06	euxassay_002173_07	euxassay_002173_08	euxassay_002173_09	euxassay_002173_10

euxassay_002173_11	euxassay_002173_12	euxassay_002173_13	euxassay_002173_14	euxassay_002173_15

euxassay_002173_16	euxassay_002173_17	euxassay_002173_18	euxassay_002173_19	euxassay_002173_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 02 03 04 05 13 weak expression: see section 06 07 09

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 12 13 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6005
Entity Detected:	Qdpr, ( MGI:97836)
Sequence:	sense strand is shown >T6005 CCCACGCGTCCGCTCGAAGTGCGTTTGGGCTCGCCGTTCAGTACCGCGCGAGTCTGTCAGGAGCAGGATG GCGGCTTCAGGCGAGGCGCGCCGGGTGCTGGTGTACGGCGGCAGGGGCGCTCTGGGCTCGCGCTGCGTAC AGGCCTTCCGGGCCCGCAACTGGTGGGTTGCCAGCATCGATGTGGTGGAGAACGAAGAGGCCAGTGCCAG CGTGGTTGTTAAGATGACAGATTCATTCACAGAGCAGGCTGACCAGGTCACTGCTGATGTTGGAAAGCTC CTAGGTGACCAGAAGGTGGATGCAATTCTCTGTGTGGCTGGAGGATGGGCCGGGGGCAATGCCAAATCCA AGTCGCTTTTTAAAAACTGTGACATGATGTGGAAGCAGAGTATGTGGACATCCACTATCTCCAGCCACTT GGCCACAAAGCACCTGAAGGAAGGAGGCCTCTTGACCCTGGCTGGGGCCAAAGCTGCCTTGGATGGGACT CCTGGGATGATCGGCTATGGCATGGCCAAGGGAGCCGTCCATCAGCTCTGCCAGAGCCTGGCAGGGAAGA ACAGTGGCATGCCCCCTGGGGCTGCTGCCATTGCTGTGCTCCCCGTTACCCTGGATACCCCGATGAACAG GAAATCAATGCCTGAGGCAGACTTCAGCTCCTGGACACCCTTAGAGTTCCTGGTGGAAACCTTCCATGAC TGGATCACTGGGAACAAACGGCCAAACTCAGGAAGCCTAATCCAGGTGGTAACCACAGATGGGAAGACAG AGCTTACTCCAGCATATTTCTAAGCTGCATCTCAGTGCCTGGGAGAGCCCTCTCGAAACATCACTAACCT GTCTGTATTGCCGAATCTGGCCCCATGTTTTCTGTATTCCTGTTTGTGGTAGGAGATAACCAGTCTTGTT TGTTTCTTACATGGCATTCCTTTGTTGTCCTGGGATGGTGAGGTATGTTTATGGGAAATGAGCAGAGGCC TGTAGGTAGCACCTGAGGGTCCAGGGAGGTATATGGACAGGCCTGCATCACAGCATCCTAGACTTCTTTG GAGACCCGTTTGATAGTACTTTCAGTTTTTGGCATTTGGGGTACTTTGAAATCCAACTTGTTTTTAAATT CCATGTTGGTGCCATAGATGTAGTTTTTACAGCCTGAAAATTAACTGCCATGAACATTCTTTGGTGATGT AAGCAAGTAAAACCCAATGGACTCTGTTGTGATATCCTTTTGTGTTCCAGCCTAGGCTAAGTGTTTGTGA AAGCCATTTGTGAGTGCTTGGAGTGTTCTTCCTAATAAACACACTTCATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3487104 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487104 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002173 same experiment
	EMAGE:29860 same embryo
	EMAGE:32091 same embryo
	EMAGE:29863 same embryo
	EMAGE:29887 same embryo
	EMAGE:32137 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source