EMAGE mouse gene expression spatial database

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EMAGE:32160

L1cam L1 cell adhesion molecule ( MGI:96721)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009800_01	euxassay_009800_02	euxassay_009800_03	euxassay_009800_04	euxassay_009800_05

euxassay_009800_06	euxassay_009800_07	euxassay_009800_08	euxassay_009800_09	euxassay_009800_10

euxassay_009800_11	euxassay_009800_12	euxassay_009800_13	euxassay_009800_14	euxassay_009800_15

euxassay_009800_16	euxassay_009800_17	euxassay_009800_18	euxassay_009800_19	euxassay_009800_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 18 19 weak expression: see section 20

ventral grey horn

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 17 weak expression: see section 06 15 16

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08

alar columns

moderate

homogeneous

moderate expression: see section 07 09 10 11 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 14

forebrain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 moderate expression: see section 16

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 16 17

trigeminal v nerve

strong

regional

strong expression: see section 06 07 13

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11

midgut

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 16 17 18 19

midbrain

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 18 moderate expression: see section 16

vagus x ganglion

strong

regional

strong expression: see section 07 15

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 14 15 16 17 18 19 moderate expression: see section 20

cervical ganglion

moderate

regional

moderate expression: see section 08 14

hindbrain

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 moderate expression: see section 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30667
Entity Detected:	L1cam, L1 cell adhesion molecule ( MGI:96721)
Sequence:	sense strand is shown >T30667 CCTATGCCAATGGAACGCTGAGCATCAGAGACCTCCAGGCCAATGACACTGGACGCTATTTCTGCCAGGC TGCCAATGACCAGAACAATGTGACCATTTTGGCTAACCTACAGGTTAAAGAAGCAACCCAGATCACACAG GGGCCCCGGAGCGCAATTGAGAAGAAAGGTGCAAGGGTGACATTCACGTGCCAGGCCTCCTTTGACCCCT CTTTGCAGGCCAGCATCACTTGGCGTGGAGATGGGAGAGACCTACAGGAACGTGGGGACAGTGACAAGTA TTTCATAGAAGATGGGAAACTAGTCATCCAGAGCCTGGACTACAGTGACCAGGGCAACTACAGTTGTGTG GCCAGCACTGAACTGGATGAGGTGGAGAGCAGGGCACAGCTCTTAGTGGTGGGGAGCCCTGGGCCAGTGC CTCACCTGGAGCTGTCCGACCGCCACCTGCTGAAGCAGAGCCAGGTGCACTTGTCTTGGAGCCCTGCTGA AGACCACAACTCTCCCATTGAGAAGTATGACATTGAATTTGAGGACAAGGAAATGGCTCCTGAGAAATGG TTCAGTCTGGGCAAGGTGCCAGGAAATCAGACCTCTACTACCCTCAAGCTGTCCCCCTATGTCCACTACA CCTTTCGGGTCACTGCCATTAACAAATATGGTCCTGGAGAACCCAGCCCTGTCTCTGAGACTGTGGTCAC ACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGATGTGAGAGGGGAAGGGAATGAGACCAACAATATGGTC ATCACATGGAAGCCCCTTCGGTGGATGGATTGGAATGCCCCCCAGATTCAGTACCGTGTACAGTGGCGTC CACAGGGCAAGCAGGAGACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6822217), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59661. Forward Primer - name:059661_F_IRAV117_c10_L1cam, sequence:CCTATGCCAATGGAACGC; Reverse Primer - name:059661_R_SP6_IRAV117_c10_L1cam, sequence:AGGTCTCCTGCTTGCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009800 same experiment
	EMAGE:30847 same embryo
	EMAGE:30527 same embryo
	EMAGE:30161 same embryo
	EMAGE:30733 same embryo
	EMAGE:29745 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source