EMAGE mouse gene expression spatial database

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EMAGE:32161

Olfm1 olfactomedin 1 ( MGI:1860437)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003026_01	euxassay_003026_02	euxassay_003026_03	euxassay_003026_04	euxassay_003026_05

euxassay_003026_06	euxassay_003026_07	euxassay_003026_08	euxassay_003026_09	euxassay_003026_10

euxassay_003026_11	euxassay_003026_12	euxassay_003026_13	euxassay_003026_14	euxassay_003026_15

euxassay_003026_16	euxassay_003026_17	euxassay_003026_18	euxassay_003026_19	euxassay_003026_20

euxassay_003026_21	euxassay_003026_22	euxassay_003026_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17 18

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 14 15 16 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 15

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 21 22

not examined

regional

not examined expression: see section 01 02 03 04 23

lower jaw molar

moderate

regional

moderate expression: see section 07 08 20

midbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 09 18

upper jaw molar

moderate

regional

moderate expression: see section 07 08 20

diencephalon

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 weak expression: see section 08 19

telencephalon marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

cervical ganglion

moderate

regional

moderate expression: see section 10 17 18

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23

hindbrain

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 23

extrinsic ocular muscle

moderate

regional

moderate expression: see section 06 21 22 weak expression: see section 02 03 04 05 20

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 17 18 19 20

nucleus pulposus

moderate

regional

moderate expression: see section 13 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 13 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4720
Entity Detected:	Olfm1, olfactomedin 1 ( MGI:1860437)
Sequence:	sense strand is shown >T4720 CTGCCCGGGGCGCGGGAGCCGGCGCCGGCGCGGCGCGGGCGCAGGAGGCCACTCGAGGCGCGATGTCGGT GCCGCTGCTGAAGATCGGGGTCGTGCTAAGCACCATGGCCATGATCACCAACTGGATGTCCCAGACGCTG CCCTCGCTGGTGGGCCTCAATACCACCCGGCTTTCGGCCGCCAGCGGCGGGACGCTGGACCGCAGCACCG GCGTGTTGCCCACCAACCCCGAGGAGAGCTGGCAGGTGTACAGCTCTGCCCAGGACAGTGAGGGCAGGTG TATCTGCACAGTGGTCGCCCCCCAGCAGACCATGTGTTCGCGGGATGCCCGCACAAAACAGCTGAGGCAG CTACTGGAGAAGGTGCAAAACATGTCTCAATCCATAGAAGTCTTGGACAGGCGAACTCAGAGAGACCTGC AGTACGTGGAGAAGATGGAGAACCAGATGAAAGGCCTGGAGACCAAGTTCAAGCAGGTGGAGGAGAGCCA TAAGCAGCATCTAGCCAGGCAGTTCAAGGCAATAAAAGCGAAAATGGATGAACTTAGGCCTTTGATTCCT GTGTTGGAAGAGTACAAGGCCGATGCCAAATTGGTATTGCAGTTTAAGGAGGAGGTCCAGAATCTGACGT CAGTGCTTAACGAACTGCAAGAGGAGATTGGCGCCTATGACTACGATGAACTTCAGAGCAGAGTGTCCAA TCTTGAAGAAAGGCTCCGTGCATGCATGCAAAAACTAGCCTGCGGGAAGCTGACAGGCATAAGTGACCCA GTGACTGTCAAGACCTCTGGCTCAAGGTTCGGGTCCTGGATGACAGACCCGCTGGCCCCAGAAGGCGATA ACCGGGTCTGGTACATGGATGGTTATCACAACAACCGCTTTGTCCGTGAGTACAAGTCCATGGTGGACTT CATGAACACGGACAACTTTACCTCTCACCGTCTCCCACACCCCTGGTCAGGTACGGGCCAGGTGGTCTAC AATGGCTCCATCTATTTCAACAAATTCCAGAGTCACATTATCATTAGGTTTGACCTGAAGACAGAGACGA TTCTGAAGACGCGCAGCCTGGACTATGCTGGCTACAACAATATGTATCACTACGCCTGGGGCGGCCACTC AGACATCGACCTCATGGTGGACGAGAATGGGCTCTGGGCTGTCTATGCCACAAACCAGAACGCAGGCAAC ATTGTCATCAGCAAGCTGGATCCTGTCTCACTGCAGATCCTCCAGACCTGGAACACCAGCTACCCCAAGC GCAGTGCCGGTGAGGCCTTCATCATCTGCGGCACCCTCTATGTCACCAACGGTTACTCGGGGGGCACCAA GGTCCACTATGCCTACCAAACCAACGCCTCGACCTACGAGTACATTGACATCCCCTTCCAGAACAAATAC TCTCACATCTCCATGCTGGACTACAACCCTAAAGACCGCGCGCTGTACGCCTGGAACAATGGCCATCAGA CCCTCTATAATGTCACCCTCTTCCATGTCATCCGCTCCGATGAGTTGTAGCTCCCGCTTTTCCAGAAGCC AAGAGTCCAAGTCCTCACCCGAGGACATTTCCATGAAACAGCTGCCAAATGATTCCAAGAATACTAACAG TCCTGATTTTGAGAAATCAGCGCAATTTTGACGCCGAGAGATGGTGTCCTCTCTGTGTTCCTCCTACTCC TGCAGGTGGGCCGCAGATTTCAGAAGAAACCGCCATGTATGTGGATGGAACTGCAGCCCAAGGCTCCTTG GCTGTCCCCATCCCACCCCATCCCTCTCTGGTCCAATAACTCAAAGAAGCAAGGCGATGACTGTTGGCCC CTCTCACCCAAGAACAACCCACTGTTAGGATTGCATGGACATTTCCTTAGGTTGTGCCCAGCGAGATGAT GTGATGGCCATTCTCCCCAACCAGCTCCCCCAGCCCCCGAGGCGCTCGCTCAGTGTAGGCTGCTTCAGCC CACACATATCGCAGCTGCCTTTTTCTTGACCGTGTTGTTGTCTCTTAGATTAACTGTGCTGAGACTCCCG GTAGCTCATGGACCCGTGTCTAGTACTTTCCCGAAGCGATGGTGGGTGTGCGTAGGTGAAGTTGCTGTAC CCACGTTGTTGAACTTGCGTACCCTGTAGCTAGATCGTGAGGTGTCCGTCTGTTATCCCCCAAGGTGGTA ACTCCGTGTGTTCAATTTATGCAATGAATGTTGTCATGCAATGCTGTAGTTTGGATTGATAAGTGGATGG TTTCTGTTTCTAAAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5036675 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5036675 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003026 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source