EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:32162

Shf Src homology 2 domain containing F ( MGI:3613669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003020_01	euxassay_003020_02	euxassay_003020_03	euxassay_003020_04	euxassay_003020_05

euxassay_003020_06	euxassay_003020_07	euxassay_003020_08	euxassay_003020_09	euxassay_003020_10

euxassay_003020_11	euxassay_003020_12	euxassay_003020_13	euxassay_003020_14	euxassay_003020_15

euxassay_003020_16	euxassay_003020_17	euxassay_003020_18	euxassay_003020_19	euxassay_003020_20

euxassay_003020_21	euxassay_003020_22	euxassay_003020_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 09 16 moderate expression: see section 08 10 11 12 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 20 21

cerebral cortex marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 moderate expression: see section 03 22

medulla oblongata basal plate

moderate

regional

moderate expression: see section 08 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

cervical ganglion

strong

regional

strong expression: see section 16 moderate expression: see section 09

metencephalon basal plate

weak

regional

weak expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 16 moderate expression: see section 10 11

rest of cerebellum marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4191
Entity Detected:	Shf, Src homology 2 domain containing F ( MGI:3613669)
Sequence:	sense strand is shown >T4191 CGAACGCGTGGGAAAGGATCCAGGATGTAGCATCTGAGCTGTCCCATGGGCTGACCTAGGCTCCTCATTA TTCAGAAATCAATCAACTCCCAACCATCTAGGAACAGAATTTGAGAGATCCCAGAGTATTCCCCGAGGAA AAGCGAAGCCAGACAAGTTGGCAATCCTAGAGGACTATGCGGACCCATTTGATGTTCAGGAGACTGGTGA AGGCTCAGGAGGGACTTCAGGAGCCCCGGGGAAGGTCCCTGAGAATGATGGCTACATGGAGCCCTACGAG GCCCAAAAGATGATGGCTGAGATCCGGGGTTCTAAGGAGACTGCAGCTCAACCCCTACCTCTCTATGACA CACCCTATGAGCCAGAAGATGAGGGGGCCAGCCCAGAAGGTGAGGGGACTCCCTGGCCCAGGGAGTCGCG CCTGCCAGAGGATGACGAGAGGCCTCCAGAAGAGTATGACCAGCCCTGGGAGTGGAAGAAGGAACGGATT TCCAAAGCCTTTGCTGCCCAGTTTGAAGGATCGGAGAAGAACTGCCTGTCACCTGGCCGGGAGGAGAAGG GGCGGCTACCTCCCAGGCTATCTGCTGGGAACCCCAAGTCAGCCAAGCCCCTAGGCATGGAACCCAGCAG CCCCCTGGGGGAGTGGACAGATCCAGCACTGCCTCTGGAAAACCAGGTCTGGTACCACGGGGCCATCAGC CGAACAGATGCCGAGAACCTGCTCAGGCTGTGCAAAGAGGCCAGCTACCTGGTGCGCAACAGTGAGACCA GCAAAAATGACTTCTCTCTCTCCCTCAAGAGCAGTCAGGGCTTCATGCACATGAAGCTGTCCCGGACTAA GGAACACAAGTATGTGTTGGGCCAGAACAGCCCACCCTTCAGCAGCGTCCCTGAAATCGTGCACCACTAT GCCAGCCGCAAGCTGCCCATTAAGGGAGCGGAACACATGTCCCTGCTCTACCCTGTGGCCATCCGGACTC TGTAGATGTGAGACCAGGGCACTATGATAGACCTGGACCCAGTCCTTGTCACCTGCACGGGGGCAGCGGT TCTTACCAAGGACATGATGTCAGAAACCTGGCTTCCCCTTGTCCTAGGATCTAGCAGGCGCTGAAGATTC CTTAATTTATTATAAAGGGGAGGGAATCCTGGGCCTTGGAATAAAGGGACTGTGTGGAGCTGGGCACCGA GGAGAGCCTGAGGAGGAAGGGGTCTCCCCGCCCCAGTCAGACACTAGGGAGGGAGTGGCGACCTCCCTCC CGCACCACCCACCCTCCGCATCCTCCCTTCTCCCTGTCCCCAGCGGGCCGCCCGGGCTCCGGGCCAGAGG CTCGGCCAGGCAGAGCTGCGGCTGCCGGCGCCGCCCAGGGGCTGCCGGGAGGGGGCGGGGAGCGAGCCCG ACTATAAATTGAGGCTGGAGCCGACGCCGGCCGCCTGCGAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4488005 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4488005 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003020 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source