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EMAGE:32163

Tyrobp TYRO protein tyrosine kinase binding protein ( MGI:1277211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32163 EMAGE:32163 EMAGE:32163 EMAGE:32163 EMAGE:32163
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EMAGE:32163 EMAGE:32163 EMAGE:32163 EMAGE:32163 EMAGE:32163
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EMAGE:32163 EMAGE:32163 EMAGE:32163 EMAGE:32163 EMAGE:32163
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
single cellstrong expression: see section 09
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9043
Entity Detected:Tyrobp, TYRO protein tyrosine kinase binding protein ( MGI:1277211)
Sequence:sense strand is shown

>T9043
CCAGGTCCCACCAGCCCCTGGACTGTGGTGTCCAGTGCATATCTGGCCACCATGGGGGCTCTGGAGCCCT
CCTGGTGCCTTCTGTTCCTTCCTGTCCTCCTGACTGTGGGAGGATTAAGTCCCGTACAGGCCCAGAGTGA
CACTTTCCCAAGATGCGACTGTTCTTCCGTGAGCCCTGGTGTACTGGCTGGGATTGTTCTGGGTGACTTG
GTGTTGACTCTGCTGATTGCCCTGGCTGTGTACTCTCTGGGCCGCCTGGTCTCCCGAGGTCAAGGGACAG
CGGAAGGGACCCGGAAACAACACATTGCTGAGACTGAGTCGCCTTATCAGGAGCTTCAGGGTCAGAGACC
AGAAGTATACAGTGACCTCAACACACAGAGGCAATATTACAGATGAGCCCACTCTATGCCCATCAGCGGC
CTGATGCCCGGATCCGGTCATTCCAGATGCCTACTCAACAAGCCCTCTCTGGGATCAGGACTCCCGTTGG
AATACAGATCCACAGGGTACCTCCCTGAGATATCTGACATTGTACCATTTCTGTCCCCAAATAAAAGACA
GAGCAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6814478 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6814478 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005681 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS