EMAGE mouse gene expression spatial database

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EMAGE:32165

Letmd1 LETM1 domain containing 1 ( MGI:1915864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003021_01	euxassay_003021_02	euxassay_003021_03	euxassay_003021_04	euxassay_003021_05

euxassay_003021_06	euxassay_003021_07	euxassay_003021_08	euxassay_003021_09	euxassay_003021_10

euxassay_003021_11	euxassay_003021_12	euxassay_003021_13	euxassay_003021_14	euxassay_003021_15

euxassay_003021_16	euxassay_003021_17	euxassay_003021_18	euxassay_003021_19	euxassay_003021_20

euxassay_003021_21	euxassay_003021_22	euxassay_003021_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4652
Entity Detected:	Letmd1, LETM1 domain containing 1 ( MGI:1915864)
Sequence:	sense strand is shown >T4652 CCACGCGTCCGGCGTGCGCCACCACCGCCCCGCAGCATGGCCCTTTTCGTAGGCTGCAGTATAAGCTCAT TTTAAGTGAGTGGGATGGAGGGATTGGCCACATTATCAGGAACTCACCCCAGTTAAAATGGGATCTGTTT ATTTCTGTTTAGTTCCGACGAGATATCACCAAGTGTCTCTTCGTTGGCCTCATCTCCATTCCACCCTTTG CCAACTACCTGGTCTTTTTGCTAATGTACCTGTTTCCTAGGCAACTGCTAGTCAAGCATTTCTGGACCCC CAAACAACAAATTGATTTCTTGGACGTTTATCATGGTCTCCGGAGGCGGTCCCACTCGGAAGTCATCACA CATCTAAGAAGGGCCAGCACTTTTGTTTCTCATGAGAAACTCCGGAGGCAGCTGACAGACCTGTGCACCA AGGTGCAGAGTGGCACCCACCCAGCAGCACAGGACGTCCTGGCTCTCAGAGATTGCTTCTCTACCTACCC CTTGGGCTTCAGCCAGCTCCAAGCCTCGCAGATGAGAGCCTTGAGCCAGGCCATGCTCCTCACACCTTAC CTGCCCCCGCCCTTGCTGAGGCAGCGGCTAAAGAGTCACACCACCGTGATCCACCAGCTGGACAGGGCTC TGGCAAAGCTGGGGATTGGCCAGCTGACTGCACAGGAAGTGAAATCGGCTTGCTATCTTCGTGGCTTGAA CTCTACCCACATTGCTGACGACAGGTGTCGAGCCTGGCTGGGGGAATGGTTGCACATCTCCTGTAGCCTG AAAGAACCCGAGCTGTCCCTCTTGCTGCACAACGTGGTCCTGCTCTCCACCAACTACCTTGAGACAAGGC GCTGAGTGAACCCCACGGAAAGGACGGCATTGTCCTGCAGTCACCTAGTGTAGTGGTGTGGGACCAAGAA GCACCTGTCAGCTGTCTGTGCGCACCACTAGTGTGGGAGGCAGCGGAGTGGGTTCCACGGGCTTCAGAGC ACGCTACCCCCCTGGGAGCTATAGACATTCCTTCACAGCTTGAGCCTAACAAACCCATGTGTGCTAACCT GGAACTGGCAAGCATCTCTGGTACTGAACTTTTATTGTGATGTAATACTTAGGTCCTATAAAGTCAGCCT TTGCCCAGGTGGGAATCATGTGGCCTAAAATTGAGCACTGCCATGGTTTTGTTTTTAAGGTGTTTTTTGT GTGTTCCTTTTTGAGATGAGTCTCTGTTGCCCAGGCTGGCCTCCAATCCCTGTGCTCAAGGGATCCTCTC TCTCCAGACTCGGGTGCTGCGGCTGCACTGGCCTCACTGCCACGTTCTCTACAGCCGAGCCTTGTTTTCC TGAGACCCCGTGTCTCATGTAGCCGAAGAGGACCTTGAGATCTGGTCTTCCTACCCCCAGCTCCCAGGCG CTGGAACACCGGCAAGGAGCCCACGAAGCTCTGGGGTGTTGTCTTGGCTGAGTGCTTTTTACCGAGTCTT TGTGCACTAGCGGATCTCTGGTACCAAGAACAGTGCAGCTTACTCTGGGGTGAGGGCACCCATGCCTGGC CCTGCGCCAACACCCCGGCAGTGGTTTTTCTTATGCCCCCTCCCTTTTCCTTTTATGGCCTTTTTGAGAG TCTTACTGTATAACCTGGCAATCCTGACAGACAATTCTGCCACAGCCTCCTGGAGCGAGAATATAAGCAT GAGCCACACCCATCCTTTCTGGTGAGACCAAAAGTAGGGCTGTTTCTCCTCGTCACTATCATGGGCTAAC TTCCACCCCTCCTCTCAGTATTCTATGGACACTTTTTTTTTTTTAATAGTAATTTATGGGAACATTCTAA AGGAGGCGGTGAGAAAGAATGGCTGCCTGGCCCCTCCCTGCGTTTGTCCCCAGTGTCTTACTTTCCAGGC TGTCACACAGCCCCTAGGGACCTTACATGTTTGCTACCTCCACTGTGTTGCACTAAGCCATCTGTAACCA GCAGTGGCTGCCTTCTCTAGGCTGTGGGTCAAAAACGCACAAGGTACTAATTACTGCCCAGCCCTGGGAG ACCAGGAGATTTGCAATAGATAGTGAGTTGGGCTTAACTGTCGTGTGTACATCTATGCCCTGTCGTTTTG TAATAACTTACTGTAAATGCATGAAGCACTGGTTTAAGCCTAAGTAAAGACTGCCTGAAACCAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5010892 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010892 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003021 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source