EMAGE mouse gene expression spatial database

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EMAGE:32188

Rcsd1 RCSD domain containing 1 ( MGI:2676394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003001_01	euxassay_003001_02	euxassay_003001_03	euxassay_003001_04	euxassay_003001_05

euxassay_003001_06	euxassay_003001_07	euxassay_003001_08	euxassay_003001_09	euxassay_003001_10

euxassay_003001_11	euxassay_003001_12	euxassay_003001_13	euxassay_003001_14	euxassay_003001_15

euxassay_003001_16	euxassay_003001_17	euxassay_003001_18	euxassay_003001_19	euxassay_003001_20

euxassay_003001_21	euxassay_003001_22	euxassay_003001_23	euxassay_003001_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

strong

regional

strong expression: see section 13 14 15 17

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17

renal cortex

strong

regional

strong expression: see section 09 10 11 12 13 18 19 20 21 22 23

hindgut

strong

regional

strong expression: see section 15 16

midbrain meninges

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

stomach

strong

regional

strong expression: see section 07 08 09 10 11 12

diencephalon meninges

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

rectum

strong

regional

strong expression: see section 15 16

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

hindbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4728
Entity Detected:	Rcsd1, RCSD domain containing 1 ( MGI:2676394)
Sequence:	sense strand is shown >T4728 TGCCCGCTACAGCCCCGAAAGTGACCCGGGCTGGGAGCCGAGGGGACAGCGGGGATCGTGGGCTCTTAAC CCGAGAGAGCGGGCGTGTCCGCAGCCGGAGGAAGACTACCTGGAGGACATGGAGGAAAGACCCTCAGAGA CCAACTCCAATGTTGACAGCTCAGCGCAGCCTTCAGTGGCCCAGCTGGCTGGGCGCTTTCGGGAGCATGC AGCTGTGGCCAGGGAGACACCAGCCAGTAAGCCAACAAGAAGGAAACCACCCTGCTCCCTCCCCCTGTTT CCCCCCAAGGTAGAGCTGGGCCAGAACGGTGAGGAGAAATCACCATCCGGCGCCAGCCACCCACCTAAAA TCAAGGTGAAGAGCTCACCCCTGATCGAGAAGCTTCAGGCCAACTTAGCCTTTGACCCGGCAGCTCTTCT GCCTGGGGCTTCACCCAAAAGTCCTGGACTCAAGGCCATCATATCACCATTTCACAGTCCCCCTTCCACA CCCAGTAGCCCCGGCATCCGATCCCACCCAAGTGAGGCAGAAGAGGTGCCTGTGAGCTTTGACCAGCCTC CGGAAGGAACTCACCTGCCCTCTTACAATAAGGTGCGGACTAGAGGCTCAATAAAAAGACGTCCTCCCTC GCGGCGATTCCGAAGGTCTCAGTCGGACTGTGGGGATTTTAGAGATTACAGGGCTGTGGAGCCATCTCAG GAAAACGGTGCCAGGGAAGAGAATGGGGATGACGTGTTTCCTAGCAAGAGCAAGGACCCCGGGTCCCCTC AACTCAACCAGGAGGCTATGGCAGACGGGGTGGAGGGAACTCCGCGGTCTGCAGAAAAGCCACGGAGAAG GAACACGTGTAACAGCACAGAGAAGCCAGAGGAGCTGGTCAGGACCCCAGAGGAGGCGAATGCTGGAGAG AAGGTTGGACAGAATCCAGACACAGCTAGTCAGGGTCATCCAGAGGTCCAGGCGCCATCGCAAACCGGCA GCCCAGAGGCTGAGAATGGGTGCGGAAGCCCACGGGAAGAGACAGCCCCGGGAGAGCATACAGACACTGG GAAGGCCACTGAAGGGACAGCCTCTGAGGAGAGGGTAGCAGATGAAGATAGGCTCGGACAGAAAAGCCCA GACGCAAATATGCCTGAGGAGGAGGGAGTGGTCAGGGAGAAAGCCCCACAAACCTCTTCTGGAAAAGCAG AAGGTACTACTATCGCAGAGCCGGATACAAAGCAAAAGGAGGAGGCGCCTCTGGAGCCAAGCTGTAGCCC AGGGGCCGACCATGCTGCGGGAGAGATCACCAGTGAGATCCAGAATGAGAAAGCAGTCTCCATGGATGAC ATCCCCATCGAGGATACCCGAATGTGATGAAGGGCTCAGGGTACCAGTGATGGGGCCACTGGAGACATCT CCTTGGAAACAGCAGCAGTAACAGCAGCAGCAGCAGTGCATGAAGCCACTGCAGAAGCAGAATACACCAA GCATCTGGAGACAGCCCAGGATGCAGGGTGGATGGTCCCCCGTAGGCTACACCAACCGTCTCCTCACTGG AGATGAGAGCTGAGGACAGTAGAGTTTTATCAACTTGAGTTTGTGTCACCTGACGCACGACTCAAAGTTA AATATATTTAAAGCAATTTGGTGTGGTGTCTGCTCCACGACCTTCCTGTTGATCCCCAAGTCTTCCTGCA GCCAACCTCCCAACTAGGCTCTTGTTGAACTTGTGTAAAGTTATGGCCTTTTTCTATCGTTCTAATGTTT TTATGGTTCTCAGACGTAACTTAAAACTAATTTTAGGTAGATAAAGAAGGTAGTCTTGTGGAGAATAAGA TTCCCTAAGTTACCGAGACCTGAAAACATACCAAAGCCCTCACGACGACCGTTCTGCTTGCTCCAGATGG GAATCAGCTGACCAGTTTAAAAAACTCAAAGAGATATTTATTAAAACATTTAAAGTCGTGTTCCTGCCTG CTCCAAGGACAGGAACTTTTCCCTGTCCCACACAGCCTGGCTGATGAGAACAAGGGGAGGTGATTGATCC ATCTCATCCCATCCAAAGGAGAGGCAGGATTCATCCCCTCCACCCCTCAAGGTGCCACCATGTGCCTGCC TTGCTCTGAGCATGGGTTGTACTCTCCTGGAAGGTTACACTTGAACAGGAAACTGGCCGGCCTCTGGGAG GGACGTTCAGTGTAGGCAGTACAGCTTACCTTCTGAGAGCGTCTCTCTTCACTTCTCTATGAAGTTTTGT TTGTTTGCTTTTGTTTTTGTTTTTTCAAATCTCACCATGTAGACTTATATGTAGCCCAGGCTGGTTTTGA ATTAGTGATCCCCCTGCCTCTGCCTCTAGATTGTGGGTATGCATCACTGTGCCTGGTTCTCAGTAAGCAT TTCTCAACTCCAAGTTCTTCTCCCGGACTCAGGAGGCATGAGTTAGGGTCTCCAGCCTGTTTTCATCAGG AACTCCTGCTCAGTATCCTGTCCGGGGATAAAACAGGGTTTGCAGGAATATGGGTCGGTCTGATATCCAT TCCTCTGATCACCCTCCCCTCCATGAACTCCTGCTGAAGAAAGGTTAACATAAATAAATGATGAATCTTC AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5037953 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5037953 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003001 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source