Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:32191

BC034902 ( MGI:2677839)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191
euxassay_007841_01 euxassay_007841_02 euxassay_007841_03 euxassay_007841_04 euxassay_007841_05
EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191
euxassay_007841_06 euxassay_007841_07 euxassay_007841_08 euxassay_007841_09 euxassay_007841_10
EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191
euxassay_007841_11 euxassay_007841_12 euxassay_007841_13 euxassay_007841_14 euxassay_007841_15
EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191 EMAGE:32191
euxassay_007841_16 euxassay_007841_17 euxassay_007841_18 euxassay_007841_19 euxassay_007841_20
EMAGE:32191
euxassay_007841_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 15
metanephros
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 13 14 15 16 17
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 15 16 17 moderate expression: see section 05
rest of cerebellum mantle layer
strong strong
regionalstrong expression: see section 04 05 06 07 08 15 16 17 18 moderate expression: see section 09
telencephalon meninges
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20 moderate expression: see section 09 10 11 12 13 14
nose
moderate moderate
regionalmoderate expression: see section 06 07 08 09 12 13 14
testis
moderate moderate
regionalmoderate expression: see section 05 06 07 08 15 16 17 weak expression: see section 18
bladder
weak weak
regionalweak expression: see section 10 11 12
pancreas
weak weak
regionalweak expression: see section 08 09 10
lower jaw molar
moderate moderate
regionalmoderate expression: see section 07 15
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 15
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 16 moderate expression: see section 03 04 05 weak expression: see section 18
telencephalon mantle layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20 21
midbrain mantle layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14
nasal septum
moderate moderate
regionalmoderate expression: see section 09 11
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 17 moderate expression: see section 02 03 weak expression: see section 18
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08
tongue
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 17 moderate expression: see section 02 03 weak expression: see section 18
pons mantle layer
strong strong
regionalstrong expression: see section 07 08 10 11 13 14 15 16 moderate expression: see section 09
forearm rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
dorsal grey horn
moderate moderate
regionalmoderate expression: see section 11 12 13 14
hypothalamus mantle layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 15 16 moderate expression: see section 09 10 11 12 13 14
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 14 15 16
hand mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 04 17 21 weak expression: see section 20
tail mesenchyme
moderate moderate
regionalmoderate expression: see section 11 12 weak expression: see section 09 10
physiological umbilical hernia
moderate moderate
regionalmoderate expression: see section 11 12
adrenal cortex
moderate moderate
regionalmoderate expression: see section 07 08 09 14 15
diencephalon lateral wall mantle layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14
lower jaw incisor
strong strong
regionalstrong expression: see section 12
upper jaw incisor
strong strong
regionalstrong expression: see section 12 moderate expression: see section 09
lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 15 16 17 weak expression: see section 11 12 13 14 18 19 20
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3146
Entity Detected:BC034902, ( MGI:2677839)
Sequence:sense strand is shown

>T3146
TGGCCTCGAGNCAGATTCGGCACGAGGTAAGATAATGATAAGACATCTTACTTTACTTCATGTATTCTTA
TAATTATTGAAGTTTTTACCTAATCATTATTTGTATAACAATAGAGAATAAATTTGATGAAAATTGTTTC
CAAAAGGAAAAGTTTCTAAGTAAAATGCACTATATTTGGTCAGTATGTATTTATTAAGGTAAAATTTTCT
CATAGTTACTATAATGAATTAAAGTCTCATTATTCAAAATGTCAAAAGACATCACCCAGCAGCTGTTGAA
ATGCATACCATGAGGCCCCACCTAAGACCAGCCTAGCAGTGTAGTCAGCTAACATGATCCCCAAGAGAAC
CTTGGGCTTGTTACAGATACATTCCTCTAAAACATAAAGGATCTTGTTTTATGTTTCAATCACTTAAAAA
TAATTTGGAAAAACACTTAAATGTTTTTTAAATATAAAAGATAATGTGTAGAAATAAGCTTTTCACAATA
TTAACTTTTAAAATGTGAAACAGAAATCCAAAATTTTTCAGTACGTAAAGAAAT
Notes:The probe template was PCR amplified from IMAGE:1547858 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1547858 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007841 same experiment
 EMAGE:29936 same embryo
 EMAGE:29839 same embryo
 EMAGE:29938 same embryo
 EMAGE:29943 same embryo
 EMAGE:32214 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS