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EMAGE:32192

Aqp4 ( MGI:107387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32192 EMAGE:32192 EMAGE:32192 EMAGE:32192 EMAGE:32192
euxassay_002150_01 euxassay_002150_02 euxassay_002150_03 euxassay_002150_04 euxassay_002150_05
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euxassay_002150_06 euxassay_002150_07 euxassay_002150_08 euxassay_002150_09 euxassay_002150_10
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euxassay_002150_11 euxassay_002150_12 euxassay_002150_13 euxassay_002150_14 euxassay_002150_15
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euxassay_002150_21 euxassay_002150_22 euxassay_002150_23 euxassay_002150_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
pancreas
weak weak
regionalweak expression: see section 07 08 09
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 13
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 10 11 12 14 15 16 moderate expression: see section 08 09 17 18
ventral grey horn
weak weak
regionalweak expression: see section 08 09 10
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 13
stomach
strong strong
regionalstrong expression: see section 05 06 07 08 moderate expression: see section 04
pons ventricular layer
strong strong
regionalstrong expression: see section 11 12 moderate expression: see section 10 weak expression: see section 13
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4582
Entity Detected:Aqp4, ( MGI:107387)
Sequence:sense strand is shown

>T4582
TGAGTGACAGAGCTGCGGCAAGGCGGTGGGGTAAGTGTGGACATTCCTGCAGTAGAGAGAGCATCATGGT
GGCTTTCAAAGGAGTCTGGACTCAGGCTTTCTGGAAGGCAGTCTCAGCAGAATTTCTGGCCACGCTTATC
TTTGTTTTGCTCGGTGTGGGATCCACCATAAACTGGGGTGGCTCAGAAAACCCCTTACCTGTGGACATGG
TCCTCATCTCCCTTTGCTTTGGACTCAGCATTGCTACCATGGTGCAGTGCTTTGGCCACATCAGTGGTGG
CCACATCAATCCCGCTGTGACTGTAGCCATGGTGTGCACACGAAAGATCAGCATCGCTAAGTCCGTCTTC
TACATCATTGCACAGTGCCTGGGGGCCATCATTGGAGCCGGCATCCTCTACCTGGTCACACCTCCCAGTG
TGGTTGGAGGATTGGGAGTCACCACGGTTCATGGAAACCTCACCGCTGGCCATGGGCTCCTGGTGGAGTT
AATAATCACTTTCCAGTTGGTGTTCACTATTTTTGCCAGCTGTGATTCCAAACGAACTGATGTTACTGGT
TCAATAGCTTTAGCAATTGGATTTTCCGTTGCAATTGGACATTTGTTTGCAATCAATTATACTGGAGCCA
GCATGAATCCAGCTCGATCTTTTGGACCCGCAGTTATCATGGGAAACTGGGCAAACCACTGGATATATTG
GGTTGGACCAATCATGGGCGCTGTGCTGGCAGGTGCCCTTTATGAGTATGTCTTCTGTCCTGATGTGGAG
CTCAAACGTCGCCTTAAGGAAGCCTTCAGCAAAGCCGCGCAGCAGACAAAAGGGAGCTACATGGAGGTGG
AGGACAACCGGAGCCAAGTGGAGACGGAAGACTTGATCCTGAAGCCCGGAGTGGTGCATGTGATTGACAT
TGACCGTGGAGAAGAGAAGAAGGGGAAAGACTCTTCGGGAGAGGTATTGTCTTCCGTATGACTAGAGGAC
AGCACTGAAGGCAGAAGAGACTCCCTAGACCTGGCCTCAGATTTCCTGCCACCCATTAAGGAAACAGATT
TGTTATAAATTAGACACTTGCGGGTTTCTTGCTTCACACCTTGTTACACAGTTTAAATAACACATATTTT
ACTATTATCAATGGGGGGGGTGAGAAAAAGCCTATAATGGATATAAAATTTTAAAACAGAAATCTTTTTG
AATGTATCCCCAAAGCAACTATGCAACTAGTGTATTGGCTTCCCTTTCATTAATAACTGAAATTATGAAC
CAAGATCTGGTCAAGTTTTGCCGTGCAGAGCATATGGACACCTCTGTGAGGAAGCTGGCATTGTCCATCG
TCTTGACTATTGACTTCATTGGATTGATTTAAAAATGACAAAATGCAGTATGTCACAGAATCATGTGCAT
TCAGGAGAAGACATGCAGTAACTTCTTCCACGAGCTATTCCTTATTTATAAACTACCTCGGAGGGGGAAA
ACATTAGCAAGGGCCATTGCTAATACATCATTTGTATTTATATATCTGACTGTCAGAACCTAACTCTAAT
TCACTATAATCCTCTCCCCCTCAGAATCCAAGAACCCATAGGGCTAAAAGTAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4985265 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4985265 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002150 same experiment
 EMAGE:31518 same embryo
 EMAGE:31460 same embryo
 EMAGE:31827 same embryo
 EMAGE:31721 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS