EMAGE mouse gene expression spatial database

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EMAGE:32193

Synpr synaptoporin ( MGI:1919253)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003004_01	euxassay_003004_02	euxassay_003004_03	euxassay_003004_04	euxassay_003004_05

euxassay_003004_06	euxassay_003004_07	euxassay_003004_08	euxassay_003004_09	euxassay_003004_10

euxassay_003004_11	euxassay_003004_12	euxassay_003004_13	euxassay_003004_14	euxassay_003004_15

euxassay_003004_16	euxassay_003004_17	euxassay_003004_18	euxassay_003004_19	euxassay_003004_20

euxassay_003004_21	euxassay_003004_22	euxassay_003004_23	euxassay_003004_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon alar plate

strong

regional

strong expression: see section 19

metencephalon

strong

regional

strong expression: see section 18

metencephalon basal plate

strong

regional

strong expression: see section 19 20

ventral grey horn

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

olfactory cortex marginal layer

strong

regional

strong expression: see section 11 12 13 15 16 17 18

medulla oblongata

strong

regional

strong expression: see section 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 09 18 20

dorsal grey horn

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 21 22 23

midbrain

strong

regional

strong expression: see section 07 08 09 10 11 12 14 15 16 17 18 19

thymus primordium

moderate

regional

moderate expression: see section 12 13 15

diencephalon

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 20 21 22 23

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 21 22 23 24

telencephalon marginal layer

strong

regional

strong expression: see section 11 12 16 17 18 20 21

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22 23 24

hindbrain

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4355
Entity Detected:	Synpr, synaptoporin ( MGI:1919253)
Sequence:	sense strand is shown >T4355 GCTGGAACCTACAGCCGGCGCTCCCCACTCACCCCGGGTCCCGGGTGAGCCGCTCACCACCAGAAGTGGC TTCTGACCCTGGGGAGCGTAGGTCAGAGGAACTTCATTCCTGAAGGGAACTGGTTCGAGAGAAGACAGAG TACGGGAGAGCCATGGACCCTGTGAGCCAGGTGGCCTCTGCGGGCACCTTTCGGGCATTGAAGGAACCCC TTGCCTTCCTGCGAGCTCTGGAGTTGCTTTTTGCAATGTTTGCATTTGCAACATGTGGTGGCTATTCAGG AGGCCTGCGGCTGAGTGTGGACTGTGTCAATAAGACAGAAAGTAACCTCAGCATTGACATAGCGTTTGCG TACCCATTCAGGCTGCAACAGGTGACCTTTGAAGTGCCCACCTGTGAGGGAAAGGAACAGCAGAAGCTGG CATTGGTTGGAGACTCCTCATCCTCAGCAGAATTCTTTGTCACCGTGGCTGTGTTCGCCTTTCTCTACTC CCTGGCTGCCACGGTCGTGTACATTTTCTTCCAGAACAAGTACCGCGAAAACAACCGGGGCCCACTCATT GACTTTATCGTCACTGTAGTCTTTTCATTCTTGTGGCTGGTGGGTTCCTCTGCTTGGGCAAAAGGACTGT CTGATGTCAAAGTGGCAACAGATCCCAAAGAAGTCCTGTTGCTGATGTCAGCTTGCAAGCAGCCTTCCAA CAAGTGCATGGCTGTCCACAGCCCTGTCATGTCCAGCTTAAACACATCTGTGGTCTTCGGGTTTTTGAAT TTTATCCTCTGGGCTGGAAACATTTGGTTTGTTTTCAAGGAGACTGGCTGGCATTCCTCTGGACAGAGAT ACCTTTCAGACCCCATGGAGAAGCACTCCAGCAGCTACAATCAAGGACGCTACAACCAAGAGAGTTATGG GTCAAGTGGTGGGTACAGCCAGCAGGCGAATCTGGGGCCAACTTCCGATGAGTTTGGCCAACAGCCTAGT GGTCCCACTTCCTTTAACAATCAGATATAACGGAGTATCACCCAAATTCTTCTGGTGATAGCATCACCAC CCTCCATTTCAGTAGCAGAAGAATTTTTAAGAGTTTCAATCAATTATTAATGTAGAGAATGTTGAATGTA AATCAGAGATCTGTAGTCCTCATTAAGACACAAAGGCCTGGGTGGTGATAAATGGTACTTAGAGATGAGA TGTCATAAGGAGATAAATTATTCATCACAGATGGGTAATTAGAAAGTATCTTGTTATATACACAGACACT TTTATGGTCGTTTTGTATTGTGGTAAAAATAGTAGAAGCATTTGTAGCTTAAAGTCTCTAGTATGTAATG TGCATAGATTACCTTAAATAGTATACAGTTTTCGTATGTGTTTTGATGCAACAAATATTTTACTGATTTC TAGATGACTATTTGATGAATCACATCATGCATGCATTACTATTTTTTAGTTTAGGCCTTTTAGAACTGTA ACTCTGTTTTGATAATTATTCTCTATTGTTTTCACTTGATAAAACAATGTTTATATGATTTCATGCATGA ATAAGTATTTTCTCACAGAATGTTTGGCTGTATTTCTGGAGACATTTCAGTTTGCATCATGAGTTTGTTA AGCAAGTATTTAAAGACATGTATGCATGTCTTTCTAGACTTTGCAAGTTTGTCACTCTGACTTTACCCAA GTTCTGAATGCATTAGAGCTAAAGTCTGGGAGCTTTCCCTATTTGTCAATAGTGTTCTGTAAGGTGACTG CTCAACACTTCTCTGATTTATTTAAGTAAATATTTACATTCTTGTAACTTTCTATGCAGTTTCGTGTATC TTATCTTACGAGCCATGACTGATAACGTCCAATGACCCTGTGCTTGTAGAGTGGCGTTTTCTTTGCATCC AGAGAGGCAGATTTAGACACCATAGTTTAAGTAGTATTAAGAAATGAGCTATGCTGTTTTATGTGACCAA ATGAACGTTTGTTTAGTTTTGTTTATTTTTGAAGCTAATATGTTGTTCATTCTAATTTTAGAAATCTCAT GTTATAGCTTAACAGAATTACTAATTTCGATTTGCTTATACATAGATTAATAATGGCTTCTCTATGTCAC TCTATTCCCAAAGAGGGTTCTCATGAAACTCTCCACAGAACATATTCATTATTAAGAAGAAACCAATCTT CGTTTCTTCATTTTAACTCAATTGAAATAAGAATGCCCAACAGGAAGGGCAAGCTAAACATCTGTGCTTC TAGATAAAGTTGTAACTGATGTGCAAATTGTATGTGTGATCTGTGATGTTGAAAGCAGTTTAGCACAAAA TAGGCTGTCTTAAATACTATCTGTAACTAGTTTCTTAAATCCTATTGTTTATTAAATCCTGTACTGTGAT ATCTATTTGACCTCAATAAAGTTATTGCATACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527827 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527827 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003004 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source