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EMAGE:32195

Il16 interleukin 16 ( MGI:1270855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195
euxassay_005661_01 euxassay_005661_02 euxassay_005661_03 euxassay_005661_04 euxassay_005661_05
EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195
euxassay_005661_06 euxassay_005661_07 euxassay_005661_08 euxassay_005661_09 euxassay_005661_10
EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195
euxassay_005661_11 euxassay_005661_12 euxassay_005661_13 euxassay_005661_14 euxassay_005661_15
EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195 EMAGE:32195
euxassay_005661_16 euxassay_005661_17 euxassay_005661_18 euxassay_005661_19 euxassay_005661_20
EMAGE:32195
euxassay_005661_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lower jaw molar
strong strong
regionalstrong expression: see section 06 07 08 17
upper jaw molar
strong strong
regionalstrong expression: see section 06 07 18
trachea
strong strong
regionalstrong expression: see section 09
thymus primordium
strong strong
regionalstrong expression: see section 07 08 09 10 11
vibrissa
strong strong
regionalstrong expression: see section 04 05 06 07 20 21
lower jaw incisor
strong strong
regionalstrong expression: see section 10 11 12 15
upper jaw incisor
strong strong
regionalstrong expression: see section 11
pharynx
strong strong
regionalstrong expression: see section 07 08 09 10 11 12
clavicle
strong strong
regionalstrong expression: see section 07 13 moderate expression: see section 14
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3601
Entity Detected:Il16, interleukin 16 ( MGI:1270855)
Sequence:sense strand is shown

>T3601
GAGACAGACAGAGGTAGAGTCTATGTCCACAACCTTCCCTAACTCCTCAGAGGTCAGGGACCCAGGTTTG
CCTGAGTCTCCTCCCCCAGGTCAGCGACCCAGCACAAAAGCTTTGTCTCCAGACCCTCTCTTGAGACTGT
TGACTACACAATCCGAGGATACTCAAGGACCAGGTCTCAAGATGCCAAGTCAGCGGGCACGGAGCTTCCC
CCTGACCAGGACCCAGTCCTGCGAGACAAAGCTGTTGGATGAAAAGGCCAGTAAGCTTTACTCCATCAGC
AGCCAGCTATCATCTGCTGTCATGAAATCCCTGCTGTGCCTTCCATCTTCAGTCTCTTGTGGCCAGATCA
CCTGCATCCCCAAGGAGAGGGTGTCTCCAAAGTCACCTTGCAACAACAGCTCAGCTGCAGAGGGTTTTGG
TGAAGCCATGGCCTCTGACACAGGGTTCTCTCTCAACCTTTCAGAGCTGAGAGAATATTCAGAAGGTCTC
ACAGAGCCCGGGGAAACCGAGGACAGGAACCACTGTTCTTCTCAGGCC
Notes:The probe template was PCR amplified from IMAGE:3167627 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3167627 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005661 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS