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EMAGE:322

Sox2 SRY-box containing gene 2 ( MGI:98364)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:322

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:322EMAGE:322Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
322_voxel_strong_3D_1.wlz
322_voxel_moderate_3D_1.wlz
322_voxel_possible_3D_1.wlz
322_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:322_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sox2 probeA
Entity Detected:Sox2, SRY-box containing gene 2 ( MGI:98364)
Sequence:sense strand is shown

>Sox2 probeA
CGGCGGAAAACCAAGACGCTCATGAAGAAGGATAAGTACACGCTTCCCGGAGGCTTGCTGGCCCCCGGCG
GGAACAGCATGGCGAGCGGGGTTGGGGTGGGCGCCGGCCTGGGTGCGGGCGTGAACCAGCGCATGGACAG
CTACGCGCACATGAACGGCTGGAGCAACGGCAGCTACAGCATGATGCAGGAGCAGCTGGGCTACCCGCAG
CACCCGGGCCTCAACGCTCACGGCGCGGCACAGATGCAACCGATGCACCGCTACGACGTCAGCGCCCTGC
AGTACAACTCCATGACCAGCTCGCAGACCTACATGAACGGCTCGCCCACCTACAGCATGTCCTACTCGCA
GCAGGGCACCCCCGGTATGGCGCTGGGCTCCATGGGCTCTGTGGTCAAGTCCGAGGCCAGCTCCAGCCCC
CCCGTGGTTACCTCTTCCTCCCACTCCAGGGCGCCCTGCCAGGCCGGGGACCTCCGGGACATGATCAGCA
TGTACCTCCCCGGCGCCGAGGTGCCGGAGCCCGCTGCGCCCAGT
nt 376 - nt 909 of AB108673.1
Notes:This probe region covers a 530bp SacII-AccI fragment of Sox2 cDNA which was blunted and ligated into the EcoRV site of pBluescriptII KS+. It was a gift from Sekido & Sasakura.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Yasuhide Furuta, Brigid Hogan
Principal investigator:Brigid Hogan, Duke University Medical Center, 388 Nanaline Duke Bldg. Box 3709, Durham, USA NC 27710
Submitted by:Yasuhide Furuta, Department of Biochemistry and Molecular Biology, Program in Genes and Development, University of Texas MD Anderson Cancer Center, Houston, USA TX 77030
Experiment type:non-screen
Acknowledgments:The template clone for probe synthesis was a kind gift from Sekido & Sasakura.
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE