EMAGE mouse gene expression spatial database

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EMAGE:32212

Rnf32 ring finger protein 32 ( MGI:1861747)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005874_01	euxassay_005874_02	euxassay_005874_03	euxassay_005874_04	euxassay_005874_05

euxassay_005874_06	euxassay_005874_07	euxassay_005874_08	euxassay_005874_09	euxassay_005874_10

euxassay_005874_11	euxassay_005874_12	euxassay_005874_13	euxassay_005874_14	euxassay_005874_15

euxassay_005874_16	euxassay_005874_17	euxassay_005874_18	euxassay_005874_19	euxassay_005874_20

euxassay_005874_21	euxassay_005874_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 11 12

oral epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 14 15 16 17 18

stomach

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 05 06 07

rectum

moderate

regional

moderate expression: see section 13

urethra of male

strong

regional

strong expression: see section 13

midgut

weak

regional

weak expression: see section 08 09 11

bladder

strong

regional

strong expression: see section 13 moderate expression: see section 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9751
Entity Detected:	Rnf32, ring finger protein 32 ( MGI:1861747)
Sequence:	sense strand is shown >T9751 AGAAAAGGTGCAGCTGAGGGACAGGAAAGGCCGTTTTGGACTACATCTGACACGTTTCGTTATCCTTTCA ATTGTCAGTTTGCTAATACTATGGGACTATGCAGAAGAATGGAAGTTATCGTAACGTGATGTTAGAGATC GTTAAAGCCACACCACAGTGTTTCCTGATTCCTGATACAGCATGCTAAGAAATAAGGATCGTTCATCTAA CAAAGGTAACTTAGCAGTCACTGCAGTTGCCTTACAGGACCACCTATTACATGATCTTCAACTTGGACAT CTCTCGGTAGCAGATCCTTGTAAGATAAAAGCACGAAAGAAAGAGAAGAAGTCAAAATCTCTCAAAAGAG ATGCAACAGCCATCATAGATACTGGGCTTAGAAAATCTACAGAGGGTCCTAACATGGAAGATCCAGAGAA GGAATATGTTCTAGACCCCACACCACCACCATTAACTTTAGCCCAGAAACTAGGCCTCTTGCCACCTCCC CCGTTGCCACTGTCATCAGATGAGTGGGAGAGAGTGAAGCAGAGATCCCTTCTACAAGGGGACTCCATTC AGCCATGCCCAATATGTAAAGAAGAATTTGAGCTCCATCCCCAGGTGCTGCTTTCCTGCTCACATGTATT CCATAGGGCATGCCTTCAGGCTTTTGAAAAGTTCACAAATAAAAAAACCTGTCCCCTCTGTAGAAAGAAC CAATATCAAACAAGAGTGATACATGATGGGGCTCGCCTGTTCAGAGTAAAGTGTGCCACCAGGATCCAAG CCTATTGGCGAGGGTACATCGTTAGAAAGTGGTACAGAAACCTGAGGAAAATAATCCCGCCTTCGGATGC CAAATTAAGGAGAAAGTTCTTTGAGGAAAAGTTCACAGAGATCAGCCACCGAATCCTGTGCTCATACAAC ACCAACATAGATGAACTCTTCTCAGAAATTGATGTCTGCTTGGCGGTAAACCGTAGCATTCTTCAGCAGC TAGATGAAAGGTGTGGCCAGATAATCACCAAGGAGGACTGGGAGAAAATCCAAGCACAGGCTGCTCACCA TGAGATCTATGAATGTTCCATCTGCCTGACTCCACTCTCCTTTCATGGCGATGGCCGGCAAGCAGCCATA GGAACCAGCAGCCAGCGTCCCAGAGAGACAGTCCTCCTGTCCTGTGCACATCTGTTCCACAATGCCTGCC TCCTGGCTTTGGAGGAGTTTTCTTTAGGAGACAATGCCCCTTTCCATGTCTGTCCTCTCTGTCGCTCCTG CTACCAAAAGAAAATTGTTGAATGCTGAATTCATGTCCAGGAAGTTAAATGTCTGACTAGAACAAGAAAG GGGTAAAGGCTATCCTGCTTTTGGATGAGCTGCATGGGTTTGGGGTAGAGGACTACATTGCTGACTGTTC TTTGTGATTATATAGAGGAAATATAAGTTCCTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6741898 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6741898 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005874 same experiment
	EMAGE:29684 same embryo
	EMAGE:29665 same embryo
	EMAGE:29687 same embryo
	EMAGE:30633 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source