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EMAGE:32213

LOC544763 ( MGI:3613460)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_002124_01 euxassay_002124_02 euxassay_002124_03 euxassay_002124_04 euxassay_002124_05
EMAGE:32213 EMAGE:32213 EMAGE:32213 EMAGE:32213 EMAGE:32213
euxassay_002124_06 euxassay_002124_07 euxassay_002124_08 euxassay_002124_09 euxassay_002124_10
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euxassay_002124_11 euxassay_002124_12 euxassay_002124_13 euxassay_002124_14 euxassay_002124_15
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5324
Entity Detected:LOC544763, ( MGI:3613460)
Sequence:sense strand is shown

>T5324
TTAGGCAATCGACTGGGAAGACTTCGTGGCTTTCCCTCCACCAAAACCTACTTCCCACACTTGGACCTGA
GGCCAGGCTCTAGCCAGGTTAAAGCCCATGCCCAAAAGGTGGCCGATGCACTGACTCTCGCTACCCAGCA
CCTGGACGACCTGCCTGCTTCTCTGTCTGCTCTCAGTGATCTACATGCGCACAAGCTCTGTGTGGACCCT
GCTAACTTCCAGTTCTTCAGCCACTGTCTGCTGGTGACTCTCGCCAGGCACTATCCTGGAGACTTCAGCC
CAGAGATGCATGCCTCCTTGGACAAGTTTCTGGGTCACGTGACTTCGGCACTGGTCTCCAAATATCGCTA
TAGTGTCACCTAAAATCGCAAATTTGAATTTTGGGATGTTTTTAGGGTTGTTTTAGTTGGGGGGCAAGGC
AATTCGGTTTGAAAAAAGCACATACAGCTATTTTAATGAAAAGTCGGGTGGGTTAGATACCATCCCATTG
TCTTAAAGTGATGACGGATT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002124 same experiment
 EMAGE:29916 same embryo
 EMAGE:29930 same embryo
 EMAGE:29921 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS