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EMAGE:32217

Lrrc48 ( MGI:1921915)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217
euxassay_006976_01 euxassay_006976_02 euxassay_006976_03 euxassay_006976_04 euxassay_006976_05
EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217
euxassay_006976_06 euxassay_006976_07 euxassay_006976_08 euxassay_006976_09 euxassay_006976_10
EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217
euxassay_006976_11 euxassay_006976_12 euxassay_006976_13 euxassay_006976_14 euxassay_006976_15
EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217 EMAGE:32217
euxassay_006976_16 euxassay_006976_17 euxassay_006976_18 euxassay_006976_19 euxassay_006976_20
EMAGE:32217 EMAGE:32217 EMAGE:32217
euxassay_006976_21 euxassay_006976_22 euxassay_006976_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
inner ear
strong strong
regionalstrong expression: see section 02 03 04 05 20 21 22
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 12
pons ventricular layer
strong strong
regionalstrong expression: see section 12
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 12
spinal cord ventricular layer
strong strong
regionalstrong expression: see section 13 moderate expression: see section 14 15 16
choroid invagination
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 15 16 17 18 19
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 06
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 06
midbrain ventricular layer
strong strong
regionalstrong expression: see section 12 13
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 17 18 19 moderate expression: see section 12 13 15 16
diencephalon roof plate
strong strong
regionalstrong expression: see section 12 13 14
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 13
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6761
Entity Detected:Lrrc48, ( MGI:1921915)
Sequence:sense strand is shown

>T6761
GGCGGTGTTTGGTTCCCCAGCAACCAAACGAAGCGTCTACTGCAAGGAGAGACAGTCCTTAGGAAGGGAA
ACCAGTGACGAGGCTACCTGGTTGATTTGTTTGTAAAGCTTTCTGCTGCTTCTCCACATCCTACCTCTGG
TCTTCACCAAGGACACTATCATCAGGAACCGTATGTGAAGGAGGAAGCCAATTTCTCAGCGAAGATGAGC
CGGCCATATGACTCGATGGAGCCAAAAGTAATGGACGATGACATGCTCAAAGCAGCTGTCGGGGAGCAAG
GGCCCCAGGAAGAAGCTGGGCAGCTAGCCAAGCAGGAGGGCATCCTCTTCAAAGATGTCCTGTCCCTGCA
GCTGGACTTCCAGAACATCCTCCGCATCGATAACCTCTGGCAGTTTGAGAACCTGAAGAAGCTGCAACTG
AACAACAACATCATCGAGCGCATCGAGGGCCTGACGAACCTCATACACCTCGTCTGGCTGGATTTGTCCT
TTAACAACATCGAGGCCATCGAGGGCCTGGATACCCTGGTCAACCTGGAGGACCTGAGCTTGTCCAATAA
CCGGATCTCCAAGGTGGACTCTCTAGATGCCCTGGTGAAGCTGCAGGTGCTGTCACTGGGCAACAACCAG
ATTAGCAACATGATGAATATCATCTACCTGCGCCGGTTCCCGTGCCTGCGAACACTCAGTCTCGCTGGGA
ATCCCGTTTCTGAGGCAGAGGAGTACAAGATGTTCATCTATGCCTATCTTTCTGACCTCGTGTACTTGGA
CTTCCGGCGCGTTGATGAGCAGATGAGAGAGATGGCGAAGATGAAACATCAGTACAGCATTGACGAGCTG
AAGCACCGCGAGGCACAGCTGCAGATGAAGCTGGAGGAGGAGCAGGCCAAGCAGGAGAAGCTGGAGGAAC
ACAAGATGGCGTTTGTTGAGCACCTGAACGGCCCCTTCCTGTTTGACAGCATGTATTCCGAGGATGTGGA
GGGCAACAAGCTGTCCTACCTGCCTGGTGTTAGAGAGCTCCTTGAAGCCTACAAGGACAAGTTTGTCATC
ATCTGCCTGAACATTTTTGAGTATGGCCTGAATCAGCAAGAAAAACGGAAGGTAGAGTTAGACACTTTCA
ATGAGTGCATACAGGAGGCCATCCTGGAAAACCAGGACCAGGGCAAGCTCAAGGTTGCCAAGTTTGAGGA
AAAGCACTTGCTGAACTTAAATGCCATCCGGGAAGAGACTGATCTGTCCAACATCGAGAAGAAGCTGACG
GAGTGCACTGAGAGCATCGGTGAGCTGTTCAACACACTCATGATCCTGGAGATGCAGCTGGTGGAACAGC
TGGAGGAGACCATAAACATATTTGAAAGAAATATCACTGACTTGGTGGGACTATTTATTGAAAATGTCCA
AAGTCTGATTGCTCAGTGCCGGGACCTGGAGAACCATCACCATGAGAAGCTCCTGGAGATCGCCATCAAC
ACCCTGGAGAAGATCCTCAAGGGCGAGATGGACGAGGACTTACCCGATGACGTGCGGGCGCTTTTTGTTG
ACAAAGACACAATTGTTAATGCCGTTGGGGCATCACATGACATCCACCTCCTGAAGATTGACAACCGAGA
AGATGAGCTGGTGACTGGAATCAACTCTTGGTGTGCACATCTAGTAGACAAGATTCACAAAGATGAGATC
ATGAGGAACCGTAAGCGAGTGAAGGAGATCAATCAATTCGTCGACCACATGCAGAGTGAACTGGACAACC
TGGAATGTGGAGACATTATAGATTAGGTGTCAGCCATTGCCCCTGAGAGTTATGGGATTTTTTTCGTTCA
CCCCTCTCTCAACACTGTTGGTAATAATTCTCAATATAGAGAAAATTATATAGAGAAAAAAAAAAAAAAA
AA
Notes:The probe template was PCR amplified from IMAGE:3673669 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3673669 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006976 same experiment
 EMAGE:32197 same embryo
 EMAGE:31939 same embryo
 EMAGE:31828 same embryo
 EMAGE:31910 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS