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EMAGE:3230

Meox1 mesenchyme homeobox 1 ( MGI:103220)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:3230
Fig 9A(wt) of Goh et al, 1997 [PMID:9334279] . Copyright: This image is from Goh KL, Development 1997 Nov;124(21):4309-19, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Notes:
After hybridization procedures, embryos were fixed in 0.1% glutaraldehyde/4% paraformaldehyde, cleared in 50% glycerol/PBS, then 80% glycerol/PBS overnight. Whole-mount embryos were mounted in 80% glycerol/PBS and photographed using bright-field or Nomarski optics.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
head paraxial mesenchyme
detected detected
Expression was detected in the presomitic and somitic mesoderm
trunk paraxial mesenchyme
detected detected
Expression was detected in the presomitic and somitic mesoderm
unsegmented mesenchyme
detected detected
Expression was detected in the presomitic and somitic mesoderm
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1101195
Entity Detected:Meox1, mesenchyme homeobox 1 ( MGI:103220)
Sequence:sense strand is shown

>MGI:1101195
AGATAAGGACAGGAAAACAACCCATCTTCCTCCATGGGATGCATTTGGACCTATCCTGCGTGTTCCGGAA
AAAGCTTTGTGGGAAGACCTCCCAGGTTCACACACATGCGCAGCTCAGATCTCAGACCCAACTTCTGAGG
ATGCTCCTGTGAGGACTGGTGGGAAAACAGCCTCAGGCAACAGTCTCCTTGGAAGAGACCCCTGTGTGCC
TCAGTATTAGATGGTGGATCTCCCAGATCCTGCTGATGTTGCAGAAGGAGGGTCAGCAAGTATTTGAATT
TCTTGCATGGAGATCTGATTGTGAGTGTTTAAAAATAACCCCAGTTCCCTTCCCCACCCCATCAAGACAG
AAGCTGTGGAAAATGATTGTCAAATGAGATGGCAAGTTAGAGCATGTATCAGTTTTCCCCTACAGCATAT
TTCATATGTTTTTTTTTCCTAAGATTACATCAAGCTAATTGTGCAAGGTCAATTCACTTTGTAAGAAAAC
TCTCAGAGAAATAAATCAACAAAAAAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
nt 1688 - nt 2235 of NM_010791.3
Notes:The Meox1 (Mox-1) probe used in this study by Goh et al, 1997 [PMID:9334279] is indicated as that used by Candia et al, 1992 [PMID:1363541] ie. a "550 bp Mox-1 fragment" of "the sequence between the PstI site, centered at nucleotide 1640 and the 3' end of the cDNA." nt1640 in the sequence in this paper equates to nt1688 in Candia's GenBank entry NM_010791.3. The authors obtained this probe from Pierre Chambon.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Goh KL; Yang JT; Hynes RO 1997 [PMID:9334279] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9334279] Goh KL, Yang JT, Hynes RO 1997 Mesodermal defects and cranial neural crest apoptosis in alpha5 integrin-null embryos. Development (124):4309-19
 [ PMID:1363541] Candia AF, Hu J, Crosby J, Lalley PA, Noden D, Nadeau JH, Wright CV 1992 Mox-1 and Mox-2 define a novel homeobox gene Development (116):1123-36
Links:MGI:1331633 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI