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EMAGE:3233

Tgfb1 transforming growth factor, beta 1 ( MGI:98725)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:3233
Fig 5I of Mahlapuu et al, 2001 [PMID:11124112] . Copyright: This image is from Mahlapuu M, Development 2001;128():155-166, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Embryo is at the 6-10 somite stage. Age of embryo at noon of plug day not specified in reference. Image annotation: al = allantois
Expression Pattern Description
Spatial Annotation:
EMAGE:3233Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3233_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3233_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
allantois
detected detected
Expression is detected throughout the yolk sac and entire allantois
yolk sac
detected detected
regionalExpression is detected throughout the yolk sac and entire allantois
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1164693
Entity Detected:Tgfb1, transforming growth factor, beta 1 ( MGI:98725)
Sequence:sense strand is shown

>MGI:1164693
CTTGGCACTGCGCTGTCTCGCAAGGAGGCAGGACCTGAGGACTCCAGACAGCCCTGCTCACCGTCGTGGA
CACTCGATCGCTACCCGGCGTTCCTCAGACGCCCCTATTCCGGACCAGCCCTCGGGAGCCACAAACCCCG
CCTCCCGCGAAGACTTCACCCCAAAGCTGGGGCGCACCCCTTGCACGCCGCCCTCCCCCCAGCCTGCCTC
TTGAGTCCCTCGCATCCCAGGACCCTCTCTCCCCCGAGAGGCAGATCTCCCTCGGACCTGCTGGCAGTAG
CTCCCCTATTTAAGAACACCCACTTTTGGATCTCAGAGAGCGCTCATCTCGATTTTTACCCTGGTGGTAT
ACTGAGACACCTTGGTGTCAGAGCCTCACCGCGACTCCTGCTGCTTTCTCCCTCAACCTCAAATTATTCA
GGACTATCACCTACCTTTCCTTGGGAGACCCCACCCCACAAGCC
Notes:The template clone used to generate the Tgfb1 probe used in this study by Mahlapuu et al, 2001 [PMID:11124112] is described as "IMAGE clone 890715".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CD-1) or (involves: 129X1/SvJ * C57BL/6)
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Mahlapuu M; Ormestad M; Enerback S; Carlsson P, 2001 [PMID:11124112] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11124112] Mahlapuu M, Ormestad M, Enerback S, Carlsson P 2001 The forkhead transcription factor Foxf1 is required for differentiation of extra-embryonic and lateral plate mesoderm. Development (128):155-66
Links:MGI:1929434 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI