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EMAGE:3242

Myf5 myogenic factor 5 ( MGI:97252)
TS18 (10.75 dpc)
in situ hybridisation

Data Images
EMAGE:3242
Fig 4C Brohmann et al, 2000 [PMID:10603359] . This image is from Brohmann et al., Development 127: 437-45 (2000), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a section of the anterior half of the forelimb. Image annotations: Arrow points towards the dorsal limb field. Arrowhead points toward the lateral plate mesoderm. M - myotome; L - liver. Bar = 250 microns.
Expression Pattern Description
Spatial Annotation:
EMAGE:3242EMAGE:3242Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3242_voxel_strong_3D_1.wlz
3242_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3242_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
regionalExpression in differentiating cells in the dorsal and ventral forelimb.
trunk mesenchyme
detected detected
regionalExpression in the lateral plate mesoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1101192
Entity Detected:Myf5, myogenic factor 5 ( MGI:97252)
Sequence:sense strand is shown

>MGI:1101192
CCAACAGGCATCTGTCCTTGTTAATTACAGAGAGACAGTCCCAAACTCCGGGAGCTCCGCCTGGATTTGC
TGGCCTGCAGCAGCCAGGGACTGGCGTGTCTCCCTCTCTGCTGAATCCAGGTATTCCCACCTGCTTCTCT
GAAGGATGGACATGACGGACGGCTGCCAGTTCTCCCCTTCTGAGTACTTCTATGAAGGCTCCTGTATCCC
CTCACCAGAGGATGAGTTTGGGGACCAGTTTGAGCCAAGAGTAGCAGCCTTCGGAGCACACAAAGCTGAG
CTGCAGGGCTCAGACGATGAGGAGCACGTGCG
nt 159750 - nt 160061 of AC021642.14
Notes:The Myf5 probe used in this study by Brohmann et al, 2000 [PMID:10603359] is indicated as that described by Ott et al., 1991 [PMID:1652425] i.e. a "BalI-ApaLI" genomic fragment "of 311 nucleotides from nucleotides 15 to 326 relative to the putative transcriptional start site of the mouse gene: 5'-CCAACAGGCATCTGTCCTTGTTAATTACAGAGAGACAGTCCCAAACTCCGGGAGCTCCGCCTGGATTTGCTGGCCTGCAGCAGCCAGGGACTCGGTGTCTCCCTCTCTGCTGAATCCAGGTATTCCCACCTGCTTCTCTGAAGGATGGACATGACGGACGGCTGCCAGTTCTCCCCTTCTGAGTACTTCTATGAAGGCTCCTGTATCCCCTCACCAGAGGATGAGTTTGGGGACCAGTTTGAGCCAAGAGTAGCAGCCTTCGGAGCACACAAAGCTGAGCTGCAGGGCTCAGACGATGAGGAGCACGTGCG-3' "
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 129P2/OlaHsd
Age:10.75 dpc
Theiler Stage:TS18
Mutations:Lbx1tm1Cbm/Lbx1+
Preparation:section
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Brohmann H; Jagla K; Birchmeier C, 2000 [PMID:10603359] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10603359] Brohmann H, Jagla K, Birchmeier C 2000 The role of Lbx1 in migration of muscle precursor cells. Development (127):437-45
 [ PMID:1652425] Ott MO, Bober E, Lyons G, Arnold H, Buckingham M 1991 Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo. Development (111):1097-107
Links:MGI:2136981 same experiment
 MGI:2136963 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI