Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3248

Cdx1 caudal type homeobox 1 ( MGI:88360)
TS12 (8.5 dpc)
immunohistochemistry

Data Images
EMAGE:3248
Figure 4G of Meyer & Gruss, 1993 [PMID:7900985] This image is from Meyer and Gruss, Development 117:191-203 (1993), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: al - allantois; fg - foregut region; hf - headfold; lpm - lateral plate mesoderm; nt - neural tube; so - somite.
Expression Pattern Description
Spatial Annotation:
EMAGE:3248Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3248_wholemount_strong_3D_1.wlz
3248_wholemount_moderate_3D_1.wlz
3248_wholemount_weak_3D_1.wlz
3248_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3248_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future hindbrain
detected detected
regionalThe anterior expression boundary in the neural tissue was localized within the posterior hindbrain.
head somite
detected detected
trunk somite
detected detected
future spinal cord
detected detected
The anterior expression boundary in the neural tissue was localized within the posterior hindbrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1345761
Entity Detected:Cdx1, caudal type homeobox 1 ( MGI:88360)
Notes:The Cdx1 antibody used in this study by Meyer & Gruss, 1993 [PMID:7900985] was raised against peptides encoding the amino- and carboxy termini of Cdx-1. The peptides were made by FMOC-synthesis on a Milligen-Biosearch-9050 peptide synthesizer. NH2 terminus: MYVGYVLDKDSPVYPGPARPC COOH terminus: CPTNAGLLGTPSPVPVKEEFLP The amino terminus was predicted from the investigated 5' sequences of Cdx-1. For this peptide, it was necessary to place a cysteine residue at the COOH-end in order to facilitate the coupling reaction.
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Strain:(NMRI x C57BL/6)F1
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol/DMSO 4:1
Secondary Antibody:goat anti-rabbit IgG
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Meyer BI; Gruss P, 1993 [PMID:7900985] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7900985] Meyer BI, Gruss P 1993 Mouse Cdx-1 expression during gastrulation. Development (117):191-203
Links:MGI:1345976 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI