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EMAGE:3251

Notch1 Notch gene homolog 1 (Drosophila) ( MGI:97363)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:3251 EMAGE:3251 EMAGE:3251 EMAGE:3251 EMAGE:3251
Fig 4b Del Amo et al, 1992 [PMID:1425352] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 4a Del Amo et al, 1992 [PMID:1425352] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 4c Del Amo et al, 1992 [PMID:1425352] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 4d Del Amo et al, 1992 [PMID:1425352] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 4e Del Amo et al, 1992 [PMID:1425352] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.
EMAGE:3251
Fig 4f Del Amo et al, 1992 [PMID:1425352] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
These are described by the authors as transverse sections. The cartoon in Fig 4a shows the approximate planes of section. Image annotations: cm, cephalic mesenchyme; cs, condensed somite; e, endocardial cells forming inner tube of primitive heart; f, foregut; pm, presomitic mesoderm. Fig 4C Del Amo et al, 1992 [PMID:1425352] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.
Expression Pattern Description
Spatial Annotation:
EMAGE:3251EMAGE:3251EMAGE:3251EMAGE:3251EMAGE:3251Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mapping3D mapping3D mapping
EMAGE:3251
spatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3251_voxel_moderate_3D_1.wlz
3251_voxel_notDetected_3D_1.wlz
3251_voxel_strong_3D_1.wlz
3251_voxel_moderate_3D_2.wlz
3251_voxel_notDetected_3D_2.wlz
3251_voxel_strong_3D_2.wlz
3251_voxel_moderate_3D_3.wlz
3251_voxel_notDetected_3D_3.wlz
3251_voxel_strong_3D_3.wlz
3251_voxel_moderate_3D_4.wlz
3251_voxel_notDetected_3D_4.wlz
3251_voxel_strong_3D_4.wlz
3251_voxel_moderate_3D_5.wlz
3251_voxel_notDetected_3D_5.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3251_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
unsegmented mesenchyme
strong strong
heart
strong strong
Expression is detected in endocardium
trunk somite
weak weak
Expression is substantially down-regulated when paraxial mesoderm condenses to form somites
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2445989
Entity Detected:Notch1, Notch gene homolog 1 (Drosophila) ( MGI:97363)
Sequence:sense strand is shown

>MGI:2445989
AGTGCCGAATGTGAGTGGGATGGCCTAGACTGTGCTGAGCATGTACCCGAGCGGCTGGCAGCGGGCACCC
TGGTCCTGGTGGTGCTGCTTCCACCCGACCAGCTACGGAACAACTCCTTCCACTTTCTGCGGGAGCTCAG
CCACGTGCTGCACACCAACGTGGTCTTCAAGCGTGATGCGCAAGGCCAGCAGATGATCTTCCCGTACTAT
GGCCACGAGGAAGAGCTGCGCAAGCACCCAATCAAGCGCTCTACAGTGGGTTGGGCCACCTCTTCACTGC
TTCCTGGTACC
nt 4729 - nt 5019 of Z11886.1
Notes:In this study by Del Amo et al, 1992 [PMID:1425352] a probe used for RNase protection assays is described in detail as an "EcoRI - KpnI fragment of the Motch cDNA clone c195" which "encodes the last 13 amino acids of the third Notch/lin-12 repeat, as well as the succeeding 84 amino acids of the Motch gene". See Fig 1A therein for sequence. The probe used for in situ hybridisation however is simply described as "a Motch antisense probe", that was hydrolysed to an average size of 0.1kb. Editor's Note: The probe details entered into the EMAGE database are based on the description of the RNase protection probe above.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:C57BL/6
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Del Amo et al, 1992 [PMID:1425352] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1425352] Del Amo FF, Smith DE, Swiatek PJ, Gendron-Maguire M, Greenspan RJ, McMahon AP, Gridley T 1992 Expression pattern of Motch, a mouse homolog of Drosophila Notch, suggests an important role in early postimplantation mouse development. Development (115):737-44
Links:MGI:1333990 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI