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EMAGE:3253

Pax7 paired box gene 7 ( MGI:97491)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3253
Figure 5C of Daston et al., 1996 [PMID:8631247] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: fl= forelimb, so= somite.
Expression Pattern Description
Spatial Annotation:
EMAGE:3253Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3253_wholemount_strong_3D_1.wlz
3253_wholemount_moderate_3D_1.wlz
3253_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3253_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is in the medial but not the ventral dermomyotome
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17195
Entity Detected:Pax7, paired box gene 7 ( MGI:97491)
Notes:The Pax7 probe used in this study by Daston et al., 1996 [PMID:8631247] is described as a "914-bp EcoRI fragment" used by Jostes et al., 1990 [PMID:1982921] . Editor's Note: The Pax7 probe used by Jostes et al is a "319bp SmaI-StuI fragment" and not a 914bp fragment. Ambiguous information - probe sequence unknown.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:3.5% formaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Daston et al., 1996 [PMID:8631247] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8631247] Daston G, Lamar E, Olivier M, Goulding M 1996 Pax-3 is necessary for migration but not differentiation of limb muscle precursors in the mouse. Development (122):1017-27
 [ doi:10.1016/0925-4773(90)90132-6] [ PMID:1982921] Jostes B, Walther C, Gruss P 1990 The murine paired box gene, Pax7, is expressed specifically during the development of the nervous and muscular system. Mech Dev (33):27-37
Links:MGI:1338707 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI