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EMAGE:3255

Pax7 paired box gene 7 ( MGI:97491)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3255
Figure 5G of Daston et al., 1996 [PMID:8631247] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: s - somite, dm - dermomyotome, fl - forelimb. Image annotations: dm - dermomyotome; fl - forelimb; s - spinal cord.
Expression Pattern Description
Spatial Annotation:
EMAGE:3255EMAGE:3255Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3255_voxel_strong_3D_1.wlz
3255_voxel_possible_3D_1.wlz
3255_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3255_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is in the medial but not the ventral dermomyotome
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17195
Entity Detected:Pax7, paired box gene 7 ( MGI:97491)
Notes:The Pax7 probe used in this study by Daston et al., 1996 [PMID:8631247] is described as a "914-bp EcoRI fragment" used by Jostes et al., 1990 [PMID:1982921] . Editor's Note: The Pax7 probe used by Jostes et al is a "319bp SmaI-StuI fragment" and not a 914bp fragment. Ambiguous information - probe sequence unknown.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:3.5% formaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Daston et al., 1996 [PMID:8631247] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8631247] Daston G, Lamar E, Olivier M, Goulding M 1996 Pax-3 is necessary for migration but not differentiation of limb muscle precursors in the mouse. Development (122):1017-27
 [ doi:10.1016/0925-4773(90)90132-6] [ PMID:1982921] Jostes B, Walther C, Gruss P 1990 The murine paired box gene, Pax7, is expressed specifically during the development of the nervous and muscular system. Mech Dev (33):27-37
Links:MGI:1338707 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI