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EMAGE:3256

Bmp5 bone morphogenetic protein 5 ( MGI:88181)
TS12 (10 Somite no.)
in situ hybridisation

Data Images
EMAGE:3256 EMAGE:3256 EMAGE:3256 EMAGE:3256
Fig 1K Solloway & Robertson, 1999 [PMID:10079236] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1I Solloway & Robertson, 1999 [PMID:10079236] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1M Solloway & Robertson, 1999 [PMID:10079236] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1O Solloway & Robertson, 1999 [PMID:10079236] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
The approximate section planes of images K, M and O are denoted in FigI (K=a, M=b and O=c) Image annotations used: rho - rhombencephalon; mes - mesenchyme; pro - prosencephalon; fg - foregut; myo - myocardium; ec - endocardium; lpm - splanchnopleure and somatopleure components of the lateral plate mesoderm; np - notochordal plate.
Expression Pattern Description
Spatial Annotation:
EMAGE:3256EMAGE:3256EMAGE:3256EMAGE:3256EMAGE:3256Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3256_wholemount_strong_3D_4.wlz
3256_wholemount_moderate_3D_3.wlz
3256_wholemount_weak_3D_1.wlz
3256_wholemount_notDetected_3D_4.wlz
3256_voxel_moderate_3D_1.wlz
3256_voxel_notDetected_3D_1.wlz
3256_voxel_strong_3D_1.wlz
3256_voxel_moderate_3D_2.wlz
3256_voxel_notDetected_3D_2.wlz
3256_voxel_strong_3D_2.wlz
3256_voxel_notDetected_3D_3.wlz
3256_voxel_strong_3D_3.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3256_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch ectoderm
detected detected
(fig1I)
heart
detected detected
(fig1I, 1M)
lateral plate mesoderm
detected detected
(fig1I, 1O)
embryo
detected detected
regionalExpression is in the distal tip of the tailbud (fig1I)
mesenchyme
detected detected
regionalExpression is in the mesenchyme underlying the dorsal rostral neurectoderm (fig1I, 1K)
neural ectoderm
detected detected
regionalExpression is in the dorsal rostral neurectoderm (fig1I, 1K, 1M)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1333123
Entity Detected:Bmp5, bone morphogenetic protein 5 ( MGI:88181)
Notes:The probe used in this study by Solloway & Robertson, 1999 [PMID:10079236] is indicated as that originally described by Solloway et al, 1998 [PMID:9664685] ie. a "~1.0kb fragment of the Bmp5 cDNA comprised of nucleotides -33 to 998 of the pro-region (gift of David Kingsley, Stanford University)". Editor's Note: GenBank entry NM_007555.1 for Bmp5 from Kingsley et al define the pro-region as an 806nt region between nt 103-909 of NM_0007555.1. Exact end-points of probe are unclear.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10 Somite no.
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Solloway & Robertson, 1999 [PMID:10079236] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10079236] Solloway MJ, Robertson EJ 1999 Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development (126):1753-68
 [ doi:10.1002/(SICI)1520-6408(1998)22:4<321::AID-DVG3>3.0.CO;2-8] [ PMID:9664685] Solloway MJ, Dudley AT, Bikoff EK, Lyons KM, Hogan BL, Robertson EJ 1998 Mice lacking Bmp6 function. Dev Genet (22):321-39
Links:MGI:1333146 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI