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EMAGE:3257

Actc1 actin, alpha, cardiac muscle 1 ( MGI:87905)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3257 EMAGE:3257 EMAGE:3257 EMAGE:3257
Fig4C of Smart N, Hill AA, Cross JC, Riley PR (2002) [PMID:14516662] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig4A of Smart N, Hill AA, Cross JC, Riley PR (2002) [PMID:14516662] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig4B of Smart N, Hill AA, Cross JC, Riley PR (2002) [PMID:14516662] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig4D of Smart N, Hill AA, Cross JC, Riley PR (2002) [PMID:14516662] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Coronal sections. Approximate location of sections C and D shown in A. Image B is a close-up of A. Image annotations: ba, branchial arch; e, endocardium; ec, endocardial cushion; lpm, lateral plate mesoderm; lv, left ventricle; m, myocardium; my, myotome; ot, outflow tract; so, somite; vm, ventricular myocardium; vt, ventricular trabeculae. Arrows indicate relative orientations of the anterior-posterior and right-left axes.
Expression Pattern Description
Spatial Annotation:
EMAGE:3257EMAGE:3257EMAGE:3257EMAGE:3257Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3257_wholemount_strong_3D_3.wlz
3257_wholemount_possible_3D_1.wlz
3257_wholemount_notDetected_3D_3.wlz
3257_voxel_notDetected_3D_1.wlz
3257_voxel_strong_3D_1.wlz
3257_voxel_notDetected_3D_2.wlz
3257_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3257_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
primitive ventricle
detected detected
see Fig 4A, 4B.
common atrial chamber
detected detected
See Fig4a, 4b.
trunk somite
detected detected
regionalExpression is in the myotome (Fig 4A)
primitive ventricle cardiac muscle
detected detected
See Fig 4c, 4d.
outflow tract
detected detected
regionalExpression is in the wall of the truncus arteriosus (see Fig 4c)
heart
detected detected
regionalExpression is in the trabeculae (see Fig 4d).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2671252
Entity Detected:Actc1, actin, alpha, cardiac muscle 1 ( MGI:87905)
Notes:The Actc1 (alpha-cardiac actin/alphaCA) probe used in this study by Smart et al, 2002 [PMID:14516662] is described as a 0.428kb RDA (representational difference analysis) clone. The exact sequence is not reported.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Smart N, Hill AA, Cross JC, Riley PR (2002) [PMID:14516662] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(03)00093-5] [ PMID:14516662] Smart N, Hill AA, Cross JC, Riley PR. 2002 A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis Mech Dev (119 Suppl 1):S65-71
Links:MGI:2671350 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI