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EMAGE:3267

Ncam1 neural cell adhesion molecule 1 ( MGI:97281)
TS14 (16 Somite no.)
immunohistochemistry

Data Images
EMAGE:3267 EMAGE:3267 EMAGE:3267 EMAGE:3267
Fig 1A of Moase & Trasler, 1991 [PMID:1821845] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1B of Moase & Trasler, 1991 [PMID:1821845] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1C of Moase & Trasler, 1991 [PMID:1821845] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1D of Moase & Trasler, 1991 [PMID:1821845] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
A) Transverse section through the trunk region showing staining in the neuroepithelium (ne), apical area of the mesoderm (m), and little to no stain in the notochord (n), hindgut (hg), and overlying ectoderm (e). B) Region of the posterior neural tube showing recent fusion of the nueral folds. Arrow points to the concentration of N-CAM along the lateral portion of the neural tube. C) Region of opposing neural folds of the posterior neuropore with N-CAM localized in the lateral portion of the neural tube (arrow). D) Open caudal region showing uniform anti-N-Cam in the neuroepithelium (scale bar = 50 microns). Authors note that there is more antibody fluorescence in the neuroepithelium than in the surrounding tissues in B, C, and D.
Expression Pattern Description
Spatial Annotation:
EMAGE:3267EMAGE:3267EMAGE:3267EMAGE:3267EMAGE:3267Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3267_voxel_notDetected_3D_1.wlz
3267_voxel_strong_3D_1.wlz
3267_voxel_notDetected_3D_2.wlz
3267_voxel_strong_3D_2.wlz
3267_voxel_notDetected_3D_3.wlz
3267_voxel_strong_3D_3.wlz
3267_voxel_notDetected_3D_4.wlz
3267_voxel_strong_3D_4.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3267_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
regionalStaining in the neuroepithelium of the closing neural tube in the posterior region
trunk mesenchyme
detected detected
regionalStaining in the apical area of the mesoderm
notochord
weak weak
surface ectoderm
not detected not detected
homogeneous
gut
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1934839
Entity Detected:Ncam1, neural cell adhesion molecule 1 ( MGI:97281)
Notes:The anti-Ncam1 antibody used in this study by Moase & Trasler, 1991 [PMID:1821845] is described as a "rabbit anti-mouse N-CAM polyclonal antibody", "generously provided by Dr Urs Rutihauser, Case Western Reserve, Cleveland, OH".
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Age:16 Somite no.
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:Sainte Marie's fixative (99:1 95% ethanol:acetic acid)
Embedding:Tissueprep
Secondary Antibody:swine anti-rabbit IgG
Labelled with:FITC
Visualisation method:fluorescence
General Information
Authors:Moase & Trasler, 1991 [PMID:1821845] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1821845] Moase CE, Trasler DG 1991 N-CAM alterations in splotch neural tube defect mouse embryos. Development (113):1049-58
Links:MGI:1934932 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI