EMAGE mouse gene expression spatial database

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EMAGE:3270

Cfc1 cripto, FRL-1, cryptic family 1 ( MGI:109448)
TS11 (early headfold Downs & Davies)
in situ hybridisation

Data Images


Fig 9E of Shen et al, 1997 [PMID:9053319] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.	Fig 9A of Shen et al, 1997 [PMID:9053319] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.	Fig 9Bof Shen et al, 1997 [PMID:9053319] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.	Fig 9C of Shen et al, 1997 [PMID:9053319] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.	Fig 9D of Shen et al, 1997 [PMID:9053319] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Fig 9F of Shen et al, 1997 [PMID:9053319] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.	Fig 9G of Shen et al, 1997 [PMID:9053319] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

The cartoon in A shows the approximate section planes of D/G, C/F and B/E. E. F and G are higher power images of B, C and D respectively. Image annotations: (B) arrowhead = node; arrow = lateral plate mesoderm. (C) arrowhead = notochordal plate. (F) arrow = neuroectoderm. (G) arrow = neuroectoderm.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

3270_voxel_notDetected_3D_1.wlz

3270_voxel_strong_3D_1.wlz

3270_voxel_notDetected_3D_2.wlz

3270_voxel_strong_3D_2.wlz

3270_voxel_notDetected_3D_3.wlz

3270_voxel_strong_3D_3.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3270_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

node

detected

regional

Cryptic staining is restricted to the ventral mesoderm layer of the node, and is not observed in the overlying dorsal layer that is continuous with the epiblast.

neural ectoderm

detected

regional

In sections that are immediately rostral to the node, staining is found in the notochordal plate and in a narrow patch of the adjacent midline neuroectoderm.

notochordal plate

detected

regional

In sections that are immediately rostral to the node, staining is found in the notochordal plate and in a narrow patch of the adjacent midline neuroectoderm. (FigC,F) In frontal sections through more rostral regions of the notochordal plate, this midline neuroectoderm staining becomes slightly broader in extent (Fig D,G)

embryo mesoderm

detected

regional

Cryptic staining is restricted to the ventral mesoderm layer of the node, and is not observed in the overlying dorsal layer that is continuous with the epiblast. Arrow indicates expression in lateral plate mesoderm

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1334279
Entity Detected:	Cfc1, cripto, FRL-1, cryptic family 1 ( MGI:109448)
Sequence:	sense strand is shown >MGI:1334279 CAGGACTGTATAGGGTCAGCACTTCCAGCCTGGTGGTTCAGAGCTCCTGACCTGAGAGGGCTTCAACACC TGGACTCCAGGATCTTCCTTTAACCCTGCTGTCTCTGGTCCAGGCAGAGGGCAGAGACATCTTCATCTTG CAAGACTGTGCATCCTGTAACCTGCTATAGTGATTCCAAGACCTGGAGTAAAGGGTGCCTCCGGGGCTAG GATATTTGAGTTTCAACTTCTGTGGTCATCGATCCCCAAGCACAGATGAGAGCGAACTCACCAACCCAGG GTATCAGTTTGAAAATGCATCAAGCCAGGCCTCTGTTTTTGGTGACTGTCGCGCTGCAGCTCATCGGTCT GGGATACAGTTATCAGAGCGAAGGAGATGGTGCCAGAGAAGTCAGCAATATCCTCTCTCCAGTGATCCCC GGGACGACACTGGACAGAACTCTGAGTAATTCCAGCAGAAAGAATGACATTCCGGAGGGGGCGCGCCTAT GGGATTCCCTTCCTGACTCCAGCACTTTGGGAGAGAGTGCAGTCCCTGTATCCCGCTGTTGCCACAATGG CGGCACCTGTGTTCTGGGCAGTTTCTGCGTGTGCCCTGCCTATTTCACTGGTCGCTATTGCGAGCACGAC CAGAGGCGCAGAGACTGTGGTGCCCTAGGGCATGGAGCTTGGACCCTGCACAGCTGCCGCCTATGCAGGT GCATCTTCTCAGCCCTGTACTGCCTCCCACACCAAACGTTCAGCCACTGTGACCTGAAAAGCTTCCTTTC TTCAGGCGCCAGAGGATCAAGAGAATGCAGCATCCCAAGCCTCCTCCTGCTGGTGCTCTGCCTCCTCCTG CAGGGTGTGGCTGGTAAGGGCTGAGGCTCCTAAGTGCGATGATAGACTCTCCTCCTGAGCTGTCACCCTT GATTACACCACAGTGTGCCAGCAAGAAAGCTGGGTGGTGGGCATCTGACTTGGTGTTGTGTCCTGTAAAT AACAGATTCACTGGAATATGCTGGATTCTCATGCTGTACAATAAAGAGGCTTAATGGTG
	nt 1 - nt 1039 of U57720.1
Notes:	The Cfc1 (cryptic) probe used is this study by Shen et al, 1997 [PMID:9053319] is described as the "entire cDNA". The cryptic cDNA sequence U57720.1 includes both UTRs and ORF sequence and was a direct submission to GenBank from Michael Shen.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	Swiss Webster
Age:	early headfold Downs & Davies
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	sectioned wholemount

Procedures

Fixation:	4% paraformaldehyde
Embedding:	cryosection
Staining procedure:	alkaline phosphatase + NBT/BCIP in 10%PVA

General Information

Authors:	Shen MM, Wang H, Leder P (1997) [PMID:9053319] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9053319] Shen MM, Wang H, Leder P 1997 A differential display strategy identifies Cryptic, a novel EGF-related gene expressed in the axial and lateral mesoderm during mouse gastrulation. Development (124):429-42
	[ PMID:8269852] Downs KM, Davies T 1993 Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope. Development (118):1255-66
Links:	MGI:1334280 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source