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EMAGE:3271

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3271 EMAGE:3271 EMAGE:3271 EMAGE:3271
Fig 1D Kimura et al, 1997 [PMID:9374391] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1A Kimura et al, 1997 [PMID:9374391] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1B Kimura et al, 1997 [PMID:9374391] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1C Kimura et al, 1997 [PMID:9374391] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
The sections B, C and D are taken from the whole-mount stained embryo in A. Image annotations: (A) arrowhead= mesen/metencephalic junction, op= optic vesicle, ot= otic vesicle, t= telencephalon, m= mesencephalon. (B) m= mesencephalon (C) op= optic vesicle, ot= otic vesicle, t= telencephalon, hv= head veins, arrowheads point to a lack of expression in the most rostral head mesenchyme and in the mesenchyme caudal to the mesencephalon. (D) ma= mandibular arch, ha= hyoid arch.
Expression Pattern Description
Spatial Annotation:
EMAGE:3271EMAGE:3271EMAGE:3271EMAGE:3271Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3271_wholemount_strong_3D_3.wlz
3271_wholemount_moderate_3D_2.wlz
3271_wholemount_weak_3D_1.wlz
3271_wholemount_notDetected_3D_2.wlz
3271_voxel_moderate_3D_1.wlz
3271_voxel_strong_3D_1.wlz
3271_voxel_notDetected_3D_1.wlz
3271_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3271_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
otocyst
strong strong
optic vesicle
strong strong
future brain
detected detected
regionalExpression is in the rostral brain with the posterior boundary at the mesen/metencephalic junction
head mesenchyme
detected detected
regionalExpression is in the cephalic mesenchyme at the level of the dorsal mesencephalon. Expression is in the cephalic mesenchyme near the head veins (hv), but is not found in the rostral mesenchyme nor in the mesenchyme caudal to the mesencephalon (arrowheads)
1st branchial arch mandibular component
detected detected
Expression is in the mandibular arch, but not in the hyoid arch.
2nd branchial arch
not detected not detected
homogeneousExpression is not detected in the hyoid (2nd) arch.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1328382
Entity Detected:Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Notes:The probe used in this study by Kimura et al, 1997 [PMID:9374391] is indicated as being originally described by Matsuo et al, 1995 [PMID:7590242] ie. as "probe A" in Figure 1A of the latter. The exact sequence is not given.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Kimura et al, 1997 [PMID:9374391] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9374391] Kimura C, Takeda N, Suzuki M, Oshimura M, Aizawa S, Matsuo I 1997 Cis-acting elements conserved between mouse and pufferfish Otx2 genes govern the expression in mesencephalic neural crest cells. Development (124):3929-41
 [ doi:10.1101/gad.9.21.2646] [ PMID:7590242] Matsuo I, Kuratani S, Kimura C, Takeda N, Aizawa S 1995 Mouse Otx2 functions in the formation and patterning of rostral head. Genes Dev (9):2646-58
Links:MGI:1332805 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI