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EMAGE:3273

Shh sonic hedgehog ( MGI:98297)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3273 EMAGE:3273 EMAGE:3273 EMAGE:3273 EMAGE:3273
Fig 4C, Chang et al, 1994 [PMID:7720571] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4A, Chang et al, 1994 [PMID:7720571] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4D, Chang et al, 1994 [PMID:7720571] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4E, Chang et al, 1994 [PMID:7720571] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4F, Chang et al, 1994 [PMID:7720571] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
The approvimate section planes of C, D, E and F are shown on A. Image annotations: ba, branchial arch; d, dorsal; fb, forebrain; fp, floor plate; mb, midbrain; nc, notochord; op, optic vesicle; ph, pharyngeal lumen; rp, Rathke's pouch; v, ventral.
Expression Pattern Description
Spatial Annotation:
EMAGE:3273EMAGE:3273EMAGE:3273EMAGE:3273EMAGE:3273Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3273_voxel_notDetected_3D_1.wlz
3273_voxel_strong_3D_1.wlz
3273_voxel_notDetected_3D_2.wlz
3273_voxel_strong_3D_2.wlz
3273_voxel_notDetected_3D_3.wlz
3273_voxel_strong_3D_3.wlz
3273_voxel_notDetected_3D_4.wlz
3273_voxel_strong_3D_4.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3273_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
regionalPresent at the ventral midline of the neural tube caudal to the hindbrain
diencephalon
detected detected
regionalPresent in the ventral diencephalon and in a ring of cells in the ventral surface of the midbrain-diencephalic region.
future forebrain
strong strong
regionalPresent in the ventral boundary region of the midbrain/forebrain
telencephalon
detected detected
The authors state that expression is present rostral to the midbrain but that it splits and extends bilaterally
neural tube floor plate
detected detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2148101
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Chang et al, 1994 [PMID:7720571] is defined as "a 249 bp BamHI/SmaI fragment", "that extends from residues 297 to 380 within the Hhg-1 open reading frame (see Fig. 1 therein) ". Editor's note: This information is ambiguous i.e. Restriction cutting of Shh cDNA sequences with BamHI and SmaI identify only one fragment in the region of 249bp (e.g. a 251nt fragment from nt930-1180 in NM_009170.2), however this does not correspond to a region between amino acid residues 297-380 (in the example here, this fragment would extend from aa201-aa284). Therefore it is unclear which region is covered by the probe.
Chemistry:RNA
Strand:antisense
Label:P33
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Chang DT, Lopez A, von Kessler DP, Chiang C, Simandl BK, Zhao R, Seldin MF, Fallon JF, Beachy PA. 1994 [PMID:7720571] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7720571] Chang DT, Lopez A, von Kessler DP, Chiang C, Simandl BK, Zhao R, Seldin MF, Fallon JF, Beachy PA 1994 Products, genetic linkage and limb patterning activity of a murine hedgehog gene. Development (120):3339-53
Links:MGI:2137954 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI