| Data Images |
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| Figure 3I of Shimamura et al., 1995
[PMID:8575293] Copyright: This image is from Development and is
displayed with the permission of The Company of Biologists Ltd. who owns
the copyright. |
Figure 3G of Shimamura et al., 1995
[PMID:8575293] Copyright: This image is from Development and is
displayed with the permission of The Company of Biologists Ltd. who owns
the copyright. |
Figure 3J of Shimamura et al., 1995
[PMID:8575293] Copyright: This image is from Development and is
displayed with the permission of The Company of Biologists Ltd. who owns
the copyright. |
Figure 3K of Shimamura et al., 1995
[PMID:8575293] Copyright: This image is from Development and is
displayed with the permission of The Company of Biologists Ltd. who owns
the copyright. |
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| Expression pattern clarity: |
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| Find spatially similar wholemount expression patterns: |
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| Find spatially similar expression patterns: |
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| Notes: |
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Simultaneous detection of Nkx2-2 (in purple) and Shh (in brown). ***Only the Nkx2-2 expression domain has been spatially annotated in this EMAGE entry***.
G is a dissected neural tube.
Image Annotations: di, diencephalon; dt, dorsal thalamus; f, forebrain; fp, floor plate; h, hindbrain; he, heart; hf, neural plate; hp, head process; is, isthmus; m, midbrain; ma, mammillary area; me, mesencephalon; mge, medial ganglionic eminence; n, notochord; oc, optic cup; os,optic stalk; pos, postotic sulcus; pt, pretectum; r2/r3,rhombomere 2/3 boundary; rh, rhombencephalon; rt, Rathke's pouch; sc, spinal cord; sp, secondary prosencephalon; t, telencephalic vesicle; tb, tailbud; tu, tubural hypothalamus; vt, ventral thalamus; zl, zona limitans.
In G, the large arrowheads point to zona limitans, small arrowheads interrhombomeric boundaries, open arrow is the rostral limit of Shh expression at the ventral midline and small arrows point to the medial ganglionic eminence. |
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| Expression Pattern Description |
| Spatial Annotation: |
 |  |  |  | | Annotation colour key:
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strong |
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moderate |
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weak |
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possible |
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not detected |
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| 3D mapping | 3D mapping | spatial mapping | wholemount mapping | |
| View mapped 3D expression image |
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| Download individual expression domains: |
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| Download all expression domains: |
EMAGE:3281_all_domains.zip |
| Find spatially similar wholemount expression patterns: |
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| Find spatially similar expression patterns: |
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| Morphological match to the template: |
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| Text Annotation: |
| Structure | Level | Pattern | Notes |
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| central nervous system |
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detected |
| | Expression in a stripe along the entire anterior-posterior extent of the central nervous system. |
| future brain |
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detected |
| regional | Expression has a dorsal deflection at the zona limitans (large arrowhead). Expression crosses the midline just ventral to the optic stalk (large arrow). Anterior to the isthmus the expression extends to the front of the brain in a more dorsal position. |
| future hindbrain |
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detected |
| regional | Expression has dorsal deflections at the interrhombomeric boundaries (small arrowheads). |
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| Annotation Validation: |
EMAGE Editor |
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| Detection Reagent |
| Type: | in situ hybridisation probe |
| Identifier: | MGI:1277654 |
| Entity Detected: | Nkx2-2, NK2 transcription factor related, locus 2 (Drosophila) ( MGI:97347) |
| Notes: | The Nkx2-2 probe used in this study by Shimamura et al., 1995 [PMID:8575293] is of undefined sequence. |
| Chemistry: | RNA |
| Strand: | antisense |
| Label: | digoxigenin |
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| Specimen |
| Organism: | mouse |
| Age: | 10 dpc |
| Theiler Stage: | TS16 |
| Mutations: | none (wild-type) |
| Preparation: | sectioned wholemount |
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| Procedures |
| Fixation: | 4% paraformaldehyde |
| Embedding: | paraffin |
| Staining procedure: | alkaline phosphatase + NBT/BCIP |
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| General Information |
| Authors: | Shimamura et al., 1995 [PMID:8575293]
Indexed by GXD, Spatially Mapped by EMAGE |
| Submitted by: | EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU |
| Experiment type: | non-screen |
| References: | [ PMID:8575293] Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL 1995 Longitudinal organization of the anterior neural plate and neural tube. Development (121):3923-33 |
| Links: | MGI:1934636 same experiment |
| | Ensembl same gene |
| | Allen Brain Atlas same gene |
| | BioGPS same gene |
| | International Mouse Knockout Project Status same gene |
| | GEISHA Chicken ISH Database same gene |
| | EMBL-EBI Gene Expression Atlas same gene |
| | BrainStars same gene |
| | ViBrism same gene |
| Data Source |  |
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