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EMAGE:3282

Nkx2-2 NK2 transcription factor related, locus 2 (Drosophila) ( MGI:97347)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3282 EMAGE:3282 EMAGE:3282 EMAGE:3282
Fig 4B Shimamura et al., 1995 [PMID:8575293] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4A Shimamura et al., 1995 [PMID:8575293] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4C Shimamura et al., 1995 [PMID:8575293] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4D Shimamura et al., 1995 [PMID:8575293] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
A, lateral view of dissected 10.5dpc brain stained for alpha-N-catenin protein (brown) and Nkx2.2 mRNA (purple). Selected transverse sections of a similar preparation are shown in B-C; section planes are indicated in A. Image annotations: abn, anterobasal nucleus; ac, anlage of anterior commissure; cf, commissural fibers; drg, dorsal root ganglion; dvdt, dorsoventral diencephalic tract; fp, floor plate; mc, motor column; me, mesencephalon; mlf, medial longitudinal fasciculus; nV, exit point of the trigeminal nerve; os, optic stalk; poc, postoptic commissure; rh, rhombencephalon; rp, roof plate; rt, Rathke's pouch; sot, supraoptic tract; t, telencephalic vesicle; tmes, mesencephalic trigeminal tract; tpoc, tract of postopic commissure. Scale bars, 200 microns for A, and 100 microns for the others. Sections B, C and D were taken from similar specimens to that shown in A (section planes are indicated in A). Double staining for Nkx2-2 mRNA (in purple) and alpha-N-catenin protein (in brown). Only data for Nkx2-2 has been spatially mapped in this EMAGE entry. Image annotations: abn, anterobasal nucleus; ac, anlage of anterior commissure; cf, commissural fibres; dvdt, dorsoventral diencephalic tract; fp, floor plate; me, mesencephalon; mlf, medial longitudinal fasciculus; nV, exit point of the trigeminal nerve; os, optic stalk; poc, postoptic commissure; rh, rhombencephalon; rt, Rathke's pouch; sot, supraoptic tract; t, telencephalic vesicle; tmes, mesencephalic trigeminal tract; tpoc, tract of postopic commissure.
Expression Pattern Description
Spatial Annotation:
EMAGE:3282EMAGE:3282EMAGE:3282EMAGE:3282Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3282_voxel_notDetected_3D_1.wlz
3282_voxel_strong_3D_1.wlz
3282_voxel_notDetected_3D_2.wlz
3282_voxel_strong_3D_2.wlz
3282_voxel_notDetected_3D_3.wlz
3282_voxel_strong_3D_3.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3282_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
detected detected
The expression stripe is dorsal to the oculomotor nucleus and midbrain tegmentum (Fig 4A).
forebrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1277654
Entity Detected:Nkx2-2, NK2 transcription factor related, locus 2 (Drosophila) ( MGI:97347)
Notes:The Nkx2-2 probe used in this study by Shimamura et al., 1995 [PMID:8575293] is described as a "2,026bp cDNA". Exact probe end points are undefined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Shimamura K; Hartigan DJ; Martinez S; Puelles L; Rubenstein JL. 1995 [PMID:8575293] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8575293] Shimamura K, Hartigan DJ, Martinez S, Puelles L, Rubenstein JL 1995 Longitudinal organization of the anterior neural plate and neural tube. Development (121):3923-33
Links:MGI:1934636 same experiment
 EMAGE:4582 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI