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EMAGE:3286

Bmp4 bone morphogenetic protein 4 ( MGI:88180)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3286
Figure 3G of Raft et al., 2004 [PMID:15084464] . Copyright: This image is from Raft S, Development 2004;131():1801-1812 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Arrowhead denotes the position of an anterior stripe. Arrow highlights the position of posterior expression.
Expression Pattern Description
Spatial Annotation:
EMAGE:3286Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3286_wholemount_strong_3D_1.wlz
3286_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3286_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
endolymphatic appendage epithelium
detected detected
regionalThe anterior stripe of expression marks the presumptive anterior and lateral cristae. The posterior focus of expression marks the posterior crista.
rest of otocyst epithelium
detected detected
regionalThe anterior stripe of expression marks the presumptive anterior and lateral cristae. The posterior focus of expression marks the posterior crista.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:18807
Entity Detected:Bmp4, bone morphogenetic protein 4 ( MGI:88180)
Notes:The probe used in this study by Raft et al., 2004 [PMID:15084464] was "a digoxigenin-labeled RNA probe for...Bmp4... (Morsli et al., 1998 [PMID:9547240] )". Morsli et al., 1998 [PMID:9547240] describe this probe as "a 1550 bp full-length mouse BMP4 cDNA (kindly provided by Brigid Hogan, Vanderbilt University, Nashville, TN)(Jones et al., 1991 [PMID:1893873] )." Editor's Note: Jones et al describe a 1.6kb clone as well as another clone that was engineered by eliminating the 3' portion of the cDNA, encoding the TGF-beta conserved region, by digestion with SmaI, and subsequent religation. This was done to ensure to cross-reactivity with the closely related gene, BMP-2. No 1.6kb Bmp4 cDNA clone from the Hogan lab can be found in GenBank therefore exact probe sequence cannot be defined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 129/Sv * C57BL/6J * FVB * SJL
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Raft S; Nowotschin S; Liao J; Morrow BE, 2004 [PMID:15084464] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01067] [ PMID:15084464] Raft S, Nowotschin S, Liao J, Morrow BE 2004 Suppression of neural fate and control of inner ear morphogenesis by Tbx1. Development (131):1801-12
 [ PMID:9547240] Morsli H, Choo D, Ryan A, Johnson R, Wu DK 1998 Development of the mouse inner ear and origin of its sensory organs. J Neurosci (18):3327-35
 [ PMID:1893873] Jones CM, Lyons KM, Hogan BL 1991 Involvement of Bone Morphogenetic Protein-4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse. Development (111):531-42
Links:MGI:3044711 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI