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EMAGE:3294

Crabp2 cellular retinoic acid binding protein II ( MGI:88491)
TS20 (12 dpc)
in situ hybridisation

Data Images
EMAGE:3294 EMAGE:3294
Figure 9B of Ruberte et al., 1992 [PMID:1333403] . Copyright: This image is from Ruberte et al., Development 115-987 (1992), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 9B of Ruberte et al., 1992 [PMID:1333403] . Copyright: This image is from Ruberte et al., Development 115-987 (1992), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Transverse section through spinal cord at day 13.5. ad, adenohypophysis; fn, frontonasal mass; md, mandible; n, neurohypophysis; t, tongue; te, telencephalon.
Expression Pattern Description
Spatial Annotation:
EMAGE:3294EMAGE:3294Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3294_voxel_strong_3D_1.wlz
3294_voxel_moderate_3D_1.wlz
3294_voxel_possible_3D_1.wlz
3294_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3294_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
detected detected
regionalTranscripts widely distributed in the brain, but several regions showed a particularly intense signal.
adenohypophysis
detected detected
neurohypophysis
detected detected
telencephalon
detected detected
fronto-nasal process
strong strong
regionalPresent in distal frontonasal mesenchyme.
tongue
strong strong
mandibular process
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:14719
Entity Detected:Crabp2, cellular retinoic acid binding protein II ( MGI:88491)
Sequence:sense strand is shown

>MGI:14719
GAATTCCGGGGAGGATCTGTTCTGCAAAGGAGACAGCAAAGTATCTTTAGCCTAAAGGACTCAGCGTCCA
GTGTTCTAGTTGAAGATCTAAAGAGAAAGCCACCTTGCTGCCACTATGCCTAACTTTTCTGGCAACTGGA
AGATCATCCGATCGGAAAACTTTGAGGAAATGCTAAAAGCTCTGGGGGTGAACATGATGATGAGGAAGAT
CGCTGTGGCTGCAGCCTCCAAGCCAGCAGTCGAGATCAAACAGGAGAATGACACTTTCTACATCAAAACC
TCCACCACTGTGCGAACCACGGAGATTAACTTCAAGATCGGGGAGGAATTTGAGGAGCAGACCGTGGATG
GGAGACCCTGTAAGAGTTTGGTGAAATGGGAGAGTGGAAACAAAATGGTGTGCGAGCAGAGGCTTCTGAA
GGGGGAGGGCCCCAAGACCTCCTGGAGCCGAGAACTGACCAATGATGGAGAGCTGATCCTGACAATGACA
GCAGATGACGTTGTGTGCACCAGGGTCTACGTCCGAGAGTGAGTGCCTACGGGTCCAAGAACTGCCTGAG
ACGACTTCTGTGCCCGCTACAGGACACAAACCTCCCTCCCACGTCCATCTTACAAACTAGCTCTCCCCTT
ACTCCTGAGGGTTACTGCTTCCTCCAAGGCCTTTTGTTCTTTGCCTTCTCTACGCCAGAGAGGGGCAGAA
GCTCAGAACCCTCCCACCGCCATTTGCCCCTCCCAGGTCAGCAGTCCCAGCTCCATACCAGGGTCCTTCC
TGGAAGAGACTGTCTCTCTGGCCTCTACTCCTTATCCTTGTAGTCTGTGTGATTTAGAATATTTATTGGT
TAATTTTATTAAAATGTTTCCGGAATTC
nt 1 - nt 868 of M35523.1
Notes:The probe used in this study by Ruberte et al., 1992 [PMID:1333403] was "synthesized using T7-polymerase...from full length cDNAs coding for...CRABP II" and was originally described by Giguere et al., 1990 [PMID:2166951] (see Figure 1 therein).
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:12 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Ruberte E; Friederich V; Morriss-Kay G; Chambon P, 1992 [PMID:1333403] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1333403] Ruberte E, Friederich V, Morriss-Kay G, Chambon P 1992 Differential distribution patterns of CRABP I and CRABP II transcripts during mouse embryogenesis. Development (115):973-87
 [ doi:10.1073/pnas.87.16.6233] [ PMID:2166951] Giguere V, Lyn S, Yip P, Siu CH, Amin S 1990 Molecular cloning of cDNA encoding a second cellular retinoic acid-binding protein. Proc Natl Acad Sci U S A (87):6233-7
Links:MGI:2150736 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI