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EMAGE:3295

Cryaa crystallin, alpha A ( MGI:88515)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:3295
Fig7A Martinez-Morales et al, 2001 [PMID:11493524] .Copyright: This image is from Martinez-Morales JR, Development 2001;128():2019-30, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Notes:
Note: lv, lens vesicle; nr, neural retina.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lens pit
moderate moderate
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2137298
Entity Detected:Cryaa, crystallin, alpha A ( MGI:88515)
Notes:The anti-alpha-A-crystallin antibody used in this study by Martinez-Morales et al., 2001 [PMID:11493524] was a polyclonal used at 1:3000 dilution. The primary antibodies were localised using either an goat anti-mouse, -rabbit or -guinea-pig biotin-conjugated IgG (Editor's Note: it is unspecified which species the primary was raised in), followed by peroxidase-conjugated streptavidin (Jackson ImmunoResearch). Peroxidase-coupled samples were revealed using NovaRed substrate kit (Vector Laboratories).
Antibody Type:polyclonal
Specimen
Organism:mouse
Strain:involves: DBA/2 * C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:anti-primary biotin-conjugated IgG
Tertiary Antibody:peroxidase-conjugated streptavidin
Labelled with:horse radish peroxidase
Visualisation method:NovaRed
General Information
Authors:Martinez-Morales JR; Signore M; Acampora D; Simeone A; Bovolenta P, 2001 [PMID:11493524] . Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11493524] Martinez-Morales JR, Signore M, Acampora D, Simeone A, Bovolenta P 2001 Otx genes are required for tissue specification in the developing eye. Development (128):2019-30
Links:MGI:2137646 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI