Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3296

Cryaa crystallin, alpha A ( MGI:88515)
TS20 (12.5 dpc)
immunohistochemistry

Data Images
EMAGE:3296
Fig7D of Martinez-Morales et al., 2001 [PMID:11493524] .Copyright: This image is from Martinez-Morales JR, Development 2001;128():2019-30, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: L, lens; nr, neural retina.
Expression Pattern Description
Spatial Annotation:
EMAGE:3296EMAGE:3296Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3296_voxel_strong_3D_1.wlz
3296_voxel_moderate_3D_1.wlz
3296_voxel_weak_3D_1.wlz
3296_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3296_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lens vesicle
moderate moderate
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2137298
Entity Detected:Cryaa, crystallin, alpha A ( MGI:88515)
Notes:The anti-alpha-A-crystallin antibody used in this study by Martinez-Morales et al., 2001 [PMID:11493524] was a polyclonal used at 1:3000 dilution. The primary antibodies were localised with either an goat anti-mouse, rabbit or guinea-pig biotin-conjugated IgG (Editor's Note: it is unspecified which species the primary was raised in), followed by peroxidase-conjugated streptavidin (Jackson ImmunoResearch). Peroxidase-coupled samples were revealed using NovaRed substrate kit (Vector Laboratories).
Antibody Type:polyclonal
Specimen
Organism:mouse
Strain:involves: DBA/2 * C57BL/6
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:anti-primary biotin-conjugated IgG
Tertiary Antibody:peroxidase-conjugated streptavidin
Labelled with:horse radish peroxidase
Visualisation method:NovaRed
General Information
Authors:Martinez-Morales JR; Signore M; Acampora D; Simeone A; Bovolenta P, 2001 [PMID:11493524] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11493524] Martinez-Morales JR, Signore M, Acampora D, Simeone A, Bovolenta P 2001 Otx genes are required for tissue specification in the developing eye. Development (128):2019-30
Links:MGI:2137646 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI