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EMAGE:3301

En1 engrailed 1 ( MGI:95389)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3301
Figure 5E of Li & Joyner, 2001 [PMID:11748135] . Copyright: This image is from Li and Joyner, Development 128:4979-91 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3301Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3301_wholemount_strong_3D_1.wlz
3301_wholemount_moderate_3D_1.wlz
3301_wholemount_possible_3D_1.wlz
3301_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3301_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regional
future hindbrain
detected detected
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2158643
Entity Detected:En1, engrailed 1 ( MGI:95389)
Notes:The probe used in this study by Li & Joyner, 2001 [PMID:11748135] is referred to that used by Millen et al., 1995 [PMID:8575294] i.e. "a 600 bp EcoRI fragment of the 3' untranslated end of the cDNA (Joyner & Martin, 1987 [PMID:2892757] )". Editor's note: Although a restriftion map appears in Fig 1 of Joyner & Martin, there is insufficient information to define the exact and entire nucleotide sequence.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Li JY; Joyner AL, 2001 [PMID:11748135] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11748135] Li JY, Joyner AL 2001 Otx2 and Gbx2 are required for refinement and not induction of mid-hindbrain gene expression. Development (128):4979-91
 [ PMID:8575294] Millen KJ, Hui CC, Joyner AL 1995 A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum. Development (121):3935-45
 [ doi:10.1101/gad.1.1.29] [ PMID:2892757] Joyner AL, Martin GR 1987 En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis. Genes Dev (1):29-38
Links:MGI:2158723 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI