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EMAGE:3310

Fst follistatin ( MGI:95586)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:3310 EMAGE:3310
Figure 9G of Feijen et al., 1994 [PMID:7821227] . Copyright: This image is from Feijen et al., Development 120, 3621-3637 (1994)), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 9G of Feijen et al., 1994 [PMID:7821227] . Copyright: This image is from Feijen et al., Development 120, 3621-3637 (1994)), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Transverse section through eye. ep, epidermis; le, lens; sl, sclera.
Expression Pattern Description
Spatial Annotation:
EMAGE:3310EMAGE:3310Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3310_voxel_strong_3D_1.wlz
3310_voxel_moderate_3D_1.wlz
3310_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3310_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
perioptic mesenchyme
detected detected
regionalSclera in the developing eye.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23936
Entity Detected:Fst, follistatin ( MGI:95586)
Sequence:sense strand is shown

>MGI:23936
GCTTCCTCTGAGCAATATCTCTGTGGGAATGATGGAGTCACCTACTCCAGTGCCTGCCACCTGAGAAAGG
CTACCTGCCTGCTGGGCAGATCTATTGGATTAGCCTATGAGGGAAAGTGTATCAAAGCAAAGTCCTGTGA
AGATATCCAGTGCACTGGTGGGAAAAAATGTTTATGGGATTTCAAGGTTGGGAGAGGCCGGTGTTCCCTC
TGTGATGAGCTGTGCCCTGACAGTAAGTCGGATGAGCCTGTCTGTGCCAGTGACAATGCCACTTATGCCA
GCGAGTGTGCCATGAAGGAAGCTGCCTGCTCCTCAGGTGTGCTACTG
nt 625 - nt 951 of NM_006350.2
Notes:The Fst probe used in this study by Feijen et al., 1994 [PMID:7821227] was described as "a 324 nucleotide fragment, spanning amino acid residues 171-279 (Shimasaki et al., 1988 [PMID:3380788] )". Editor's note: The co-ordinates delineated above against NM_006350.2 were deduced by comparing the sequence shown in Fig2 of Shimasaki et al., 1988 and those in GenBank.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:(C57BL/6 x CBA)F1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Feijen A; Goumans MJ; van den Eijnden-van Raaij AJ, 1994 [PMID:7821227] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7821227] Feijen A, Goumans MJ, van den Eijnden-van Raaij AJ 1994 Expression of activin subunits, activin receptors and follistatin in postimplantation mouse embryos suggests specific developmental functions for different activins. Development (120):3621-37
 [ doi:10.1073/pnas.85.12.4218] [ PMID:3380788] Shimasaki S, Koga M, Esch F, Cooksey K, Mercado M, Koba A, Ueno N, Ying SY, Ling N, Guillemin R 1988 Primary structure of the human follistatin precursor and its genomic organization. Proc Natl Acad Sci U S A (85):4218-22
Links:MGI:2135921 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI