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EMAGE:3321

Ccnd2 cyclin D2 ( MGI:88314)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3321
Fig 2E Powles et al., 2004 [PMID:15031102] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2003.11.024] Dev Biol 268: 24-38, Powles N; Babbs C; Ficker M; Schimmang T; Maconochie M, Identification and analysis of genes from the mouse otic vesicle and their association with developmental subprocesses through in situ hybridization. Copyright 2004

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3321Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3321_wholemount_strong_3D_1.wlz
3321_wholemount_moderate_3D_1.wlz
3321_wholemount_possible_3D_1.wlz
3321_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3321_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
otocyst
detected detected
regionalOtic vesicle; dorsally restricted expression.
endolymphatic appendage
detected detected
Endolymphatic duct.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3505895
Entity Detected:Ccnd2, cyclin D2 ( MGI:88314)
Notes:The Ccdn2 probe used in this study by Powles et al., 2004 [PMID:15031102] was synthesised from clone np293, which was obtained through a subtractive hybridisation procedure.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 x CBA
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Powles N, Babbs C, Ficker M, Schimmang T, Maconochie M, 2004 [PMID:15031102] . Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2003.11.024] [ PMID:15031102] Powles N, Babbs C, Ficker M, Schimmang T, Maconochie M 2004 Identification and analysis of genes from the mouse otic vesicle and their association with developmental subprocesses through in situ hybridization. Dev Biol (268):24-38
Links:MGI:3510322 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI