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EMAGE:334

Wnt5a wingless-related MMTV integration site 5A ( MGI:98958)
TS21 (13.5 dpc)
in situ hybridisation

Data Images
EMAGE:334
Figure 5C of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations: arrowhead indicates head mesenchymal cells; arrows indicate postmitotic hippocampal neurons in the pyramidal cell layer; asterisk indicates shrinking cortical hem; cpe - choroid plexus epithelium.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalExpression in the cortical plate of the entire medial cerebral cortex, including the hippocampus (postmitotic neurons in the pyramidal cell layer) and adjacent limbic cortical areas. Expression marks the shrinking cortical hem.
head mesenchyme
detected detected
Expression also labels head mesenchymal cells.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2136849
Entity Detected:Wnt5a, wingless-related MMTV integration site 5A ( MGI:98958)
Notes:The Wnt5a probe used in this study by Grove et al., 1998 [PMID:9584130] is indicated as being "derived from (a) subcloned PCR fragment" and is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:13.5 dpc
Theiler Stage:TS21
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:alkaline phosphatase
General Information
Authors:Grove et al., 1998 [PMID:9584130] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9584130] Grove EA, Tole S, Limon J, Yip L, Ragsdale CW 1998 The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development (125):2315-25
Links:MGI:2136857 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE